补气活血通络法对大鼠神经吻合术后肌萎缩的影响  

Effect of Buqi Huoxue Tongluo Method on Muscle Atrophy after Rat Anastomosis

作　　者：刘俊宁[1] 王庆莲[2] 牛素生[1] 刘宇[1] 张燕[1] LIU Junning WANG Qinglian NIU Susheng LIU Yu ZHANG Yan(Fujian University of TCM, Orthopaedics＆Traumatology Faculty, Fuzhou 350122, China The Third People＇s Hospital of Fujian University of TCM, Gastroenterology, Fuzhou 350122, China)

机构地区：[1]福建中医药大学骨伤学院,福建福州350122 [2]福建中医药大学附属第三人民医院脾胃科,福建福州350122

出　　处：《云南中医学院学报》2017年第2期7-10,32,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：福建省自然科学基金面上项目(2016J01772);福建省自然科学基金(2015J01684);福建中医药大学校管课题资助(X2015004-平台);福建中医药大学校管-重点学科专项(X2014085)

摘　　要：目的研究补气活血通络法对大鼠神经吻合术后肌肉萎缩的防治作用。方法采用SPF级SD大鼠,雌雄各半,随机分为假手术组、生理盐水组、甲钴胺片组以及补阳还五汤组四组。分别在造模给药后2、4疗程(15d/疗程)取材进行大体观察和组织学观察。结果给药4疗程时补阳还五汤组大鼠跛行改善明显,手术侧肢体萎缩情况明显优于生理盐水组和甲钴胺片组;生理盐水组腓肠肌呈进行性萎缩,补阳还五汤组和甲钴胺片组于2疗程时萎缩较为明显,而4疗程时二者肌萎缩均有所改观;组织学观察2、4疗程时补阳还五汤组的肌纤维横截面积明显大于甲钴胺片组,且胶原纤维增生情况较轻;2、4疗程时补阳还五汤组和甲钴胺片组的胶原纤维含量明显少于同时期生理盐水组,且补阳还五汤组胶原纤维含量于4疗程时明显低于2疗程。结论采用补气活血通络法可明显延缓神经吻合术后的肌肉萎缩,且对肌萎缩的恢复具有治疗作用。Objective To study the preventive and therapeutic effect of Buqi Huoxue Tongluo on muscle atrophy after nerve anastomosis in rats. Methods SPF grade SD rats were randomly divided into sham operation group, saline group, Methylcobalamin group and Buyang Huanwu Decoction group. The rats were sacrificed at the end of the course of the course of treatment （15 days/course of treatment） for gross observation and histological observation. Results The treatment of limb atrophy was significantly better than that of the normal saline group and the mecobalamin group. The gastrocnemius muscle of the saline group was progressive atrophy, And mecobalamin tablets in the 2 course of treatment atrophy is more obvious, and 4 courses of both muscle atrophy have changed; histological observation of 2, 4 treatment of Buyang Huanwu Decoction group of muscle fiber cross-sectional area was significantly greater than the cobalt The content of collagen fibers in the group of Buyang Huanwu Decoction and Mecobalamin Tablets was significantly less than that in the same period, and the collagen group Fiber content in 4 courses was significantly lower than 2 courses. Conclusion Qi and blood circulation method can obviously delay the muscle atrophy after nerve anastomosis and have therapeutic effect on the recovery of muscular atrophy.

关 键 词：神经吻合术 补气活血通络法 肌萎缩 胶原纤维 功能活动 

分 类 号：R285.5[医药卫生—中药学]