靶蛋白Ezrin和Lamin A/C与卵巢癌的表达及意义相关研究  

Target protein Ezrin and Lamin A/C associated with the expression and significance of ovarian cancer research

作　　者：许海[1] 马丹凤[1] 赵井苓[1] 莫国艳[2] 

机构地区：[1]湖北中医药大学黄家湖医院妇科,湖北武汉430065 [2]湖北中医药大学重点实验室,湖北武汉430065

出　　处：《中华全科医学》2017年第6期924-926,933,共4页Chinese Journal of General Practice

基　　金：中国博士后科学基金(2013M542498);湖北中医药大学青苗计划

摘　　要：目的探讨并分析埃滋蛋白(Ezrin)和核纤层蛋白A/C抗体(Lamin A/C)在卵巢癌组织中的表达变化及其与患者临床病理特征的关系。方法选取2011年5月—2016年5月在湖北中医药大学黄家湖医院病理科收集的30例正常卵巢组织标本(正常组)、手术后切除经病理学证实的卵巢癌标本65例(卵巢癌组)及卵巢良性肿瘤组织40例(良性组),采用免疫组织化法检测3组标本中的Ezrin和Lamin A/C蛋白的表达情况,并分析其与患者的临床病理特征的关系。结果正常组、良性组的卵巢组织标本中Ezrin和Lamin A/C蛋白表达阳性率分别为93.33%、87.50%、93.33%、90.00%,显著高于卵巢癌组的49.23%、52.31%,差异均有统计学意义(P<0.05);卵巢组织标本中Ⅰ+Ⅱ期、高+中分化、未发生淋巴结转移的患者Ezrin和Lamin A/C蛋白表达阳性率显著高于Ⅲ+Ⅳ期、低分化、发生淋巴结转移的卵巢癌患者,差异均有统计学意义(P<0.05)。结论 Ezrin和Lamin A/C蛋白的表达与卵巢癌转移密切相关,Ezrin和Lamin A/C蛋白在卵巢癌组织中表达下调,并且与患者的临床病理特征具有一定的关系,同时提示了Lamin A/C以及Ezrin可以作为潜在的卵巢癌患者病情评估以及临床预后的指标。Objective To study the relationship Ezrin protein analysis（ Ezrin） and lamin A/C antibody（ Lamin A/C）changes in ovarian cancer tissues and clinicopathological features and patient. Methods Select the hospital pathology department collected 30 cases of normal ovarian tissues（ normal group）,confirmed by pathology after surgical resection specimens of 65 cases of ovarian cancer（ ovarian cancer group） and 40 cases of benign ovarian tumor（ benign group）,using immune expression were detected three groups of specimens Ezrin and Lamin A/C protein and analyze its relationship with clinical and pathological features of patients. Results In normal group,the positive expression of ovarian tissue benign group of Ezrin and Lamin A/C protein,respectively（ 93. 33%,87. 50%,93. 33%,90. 00%） was significantly higher than those in ovarian cancer group（ 49. 23%,52. 31%）. The difference was statistically significant（ P〈0. 05）. Ovarian tissue samples Ⅰ ＋ Ⅱ stage,high ＋ differentiation,Ezrin did not occur in patients with lymph node metastasis and Lamin A/C protein expression was significantly higher than that of Ⅲ ＋ Ⅳ,the positive rate of poorly differentiated ovarian cancer lymph node metastasis,The difference was statistically significant（ P〈0. 05）. Conclusion The expression of Ezrin protein and Lamin A/C are closely associated with ovarian cancer metastasis,Ezrin and Lamin A/C protein downregulated in ovarian cancer,and the clinical and pathological characteristics of patients with a certain relationship,It also suggests that Lamin A/C and Ezrin may serve as potential prognostic and prognostic indicators for patients with ovarian cancer.

关 键 词：埃滋蛋白 核纤层蛋白A/C抗体 卵巢癌组织 临床病理特征 

分 类 号：R737.31[医药卫生—肿瘤] R730.43[医药卫生—临床医学]