AngⅡ通过刺激肝癌细胞发生上皮间质转换促进肝癌转移  

Angiotensin Ⅱ induces epithelial-mesenchymal transition in hepatocellular carcinoma cells

作　　者：齐明华[1] 刘吉奎[1] 叶静[1] 李明华[1] 万汇涓[1] 漆正宇[1] 尹耀新[1] 欧希[1] 余光银[1] 

机构地区：[1]北京大学深圳医院感染性疾病科,广东省深圳市518036

出　　处：《世界华人消化杂志》2017年第12期1103-1109,共7页World Chinese Journal of Digestology

基　　金：深圳市知识创新计划基础研究基金资助项目;No.20130314100122;广东省科技计划基金资助项目;No.2016ZC0247~~

摘　　要：目的探讨肾素-血管紧张素系统(renin angiotensin system,RAS)在肝癌组织中的表达及血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)刺激肝癌细胞发生上皮间质转换现象.方法搜集56例肝癌手术标本,组织标本石蜡切片,免疫组织化学法测定血管紧张素转化酶1(angiotensin converting enzyme 1,ACE1)、ACE2、vimentin、ecadherin蛋白表达定量;将细胞分组,用AngⅡ刺激肝癌细胞系,比较不同分组细胞系vimentin、ecadherin蛋白表达差异.结果与正常组织相比,肝癌组织ACE1、AT1表达上升,上皮间质转化(epithelial mesenchymal transition,EMT)标志物vimentin蛋白表达均上升,ecadherin均下降.AngⅡ刺激下,SSMC7721细胞系的vimentin基因和蛋白表达均上升,ecadherin下降;加入AngⅡ的抑制剂Ang1-7后,这种变化受到抑制;加入Ang1-7的抑制剂A779后,再次出现vimentin上升,ecadherin表达下降.单独加入A779与对照组差别不大.结论 AngⅡ可能通过刺激肝癌细胞发生EMT对肝癌的转移起到促进作用.AIM To detect the expression of molecules of the renin-angiotensin system （RAS） in hepatocellular carcinoma （HCC） and explore whether angiotensin II （Ang II） induces epithelial-mesenchymal transition SSMC7721 cells. （EMT） in METHODS Fifty-six HCC tissues were collected, and immunohistochemistry and Western blot were used to assess the expression of angiotensin converting enzyme I （ACE1）, ACE2, vimentin, E-cadherin proteins in these tissues. Ang II was then used to stimulate SSMC7721 cells to detect whether EMT markers such as vimentin and E-cadherin were induced. RESULTS Compared with normal tissues, the expression of ACE1 and AT1 in HCC tumor tissues were higher. Ang II up-regulated the expression of vimentin but down-regulated the expression of E-cadherin in SSMC7721 cells. CONCLUSION The RAS may play a role in HCC metastasis

关 键 词：肾素血管紧张素醛固酮系统 肝癌细胞 上皮间质转换 

分 类 号：R735.7[医药卫生—肿瘤]