亚硒酸钠对肺癌A549细胞自噬相关蛋白Beclin1、LC3的作用  

EFFECT OF SODIUM SELENITE ON EXPRESSION OF AUTOPHAGY RELATED PROTEINS BECLIN1 AND LC3 IN LUNG CANCER A549 CELLS

作　　者：魏晓菲[1] 赵上[1] 王慧[1] 杜长青[1] 苏衍萍[1] 

机构地区：[1]泰山医学院组胚教研室,泰安271000

出　　处：《营养学报》2017年第2期156-160,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.81572868);山东省自然科学基金(No.ZR2011CM042);山东省自然科学基金(No.ZR2014CM023)

摘　　要：目的观察亚硒酸钠对肺癌细胞株A549细胞株自噬相关蛋白Beclin1、LC3的作用。方法将人肺癌A549细胞株分为0、0.5、2.5、5.0、8.0μmol/L亚硒酸钠共5组,用不同浓度的亚硒酸钠培养液培养24h、48h后,用免疫细胞化学染色和Western blot印迹法检测亚硒酸钠对自噬相关蛋白Beclin1、LC3表达的影响。结果人肺癌A549细胞株用不同浓度的亚硒酸钠培养24h,48h后,细胞数量逐渐减少,细胞肿胀,变大变圆,染色质凝集、核固缩并断裂。免疫细胞化学和Western blot印迹法结果均显示:随着亚硒酸钠浓度增加,肺癌A549细胞中Beclin1和LC3蛋白免疫细胞化学染色的平均光密度(MOD)有明显提高(P<0.05)。结论亚硒酸钠可以抑制肺癌A549细胞生长,诱导肺癌A549细胞自噬。Objective To study the effect of sodium selenite on the expressions of autophagy related proteins Beclinl and LC3 in lung cancer A549 cells. Methods Human lung cancer A549 cells were divided into 5 groups, and cultured with different concentrations （0, 0.5, 2.5, 5.0 and 8.0μmol/L） of sodium selenite for 24h and 48h, respectively. The expression of Beclinl and LC3 proteins was detected by immunocytochemical and Western blot methods. Results Sodium selenite decreased the number of A549 cells, induced cell swelling, and changed cells into a round shape. Chromatin condensation and nuclear fracture were also observed in a concentration and time-dependent manner. Immunohistochemical results showed that sodium selenite increased the mean optic density （MOD） of Beclinland LC3 protein in A549cells. Western blot showed that sodium selenite increased the Beclinland LC3 protein levels （P〈0.05）. Conclusion Sodium selenite can inhibit the growth of A549 lung cancer cells, and induce autophagy in A549 cells.

关 键 词：亚硒酸钠 肺癌 自噬 BECLIN1 LC3 

分 类 号：R734.2[医药卫生—肿瘤]