皮质酮对海马HT-22细胞株锌含量的影响及机制研究  

EFFECT OF CORTICOSTERONE ON ZINC CONTENT IN HIPPOCAMPAL HT-22 CELLS AND ITS MECHANISMS

作　　者：孙艳[1] 屈易萃[1] 沈志雷[1] 李红霞[1] 莫烽锋[1] 黄俊龙[1] 沈慧[1] 

机构地区：[1]第二军医大学舰艇卫生学教研室,上海204433

出　　处：《营养学报》2017年第2期161-164,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.81001243;81573150)

摘　　要：目的通过观察皮质酮(corticosterone,CORT)对海马HT-22细胞总锌含量的影响,对其可能机制进行探讨,为研究应激相关疾病提供实验依据。方法小鼠海马神经元HT-22细胞株,经10μmol/L CORT处理6h后,采用原子吸收分光光度计火焰法测定胞内总锌含量,并采用实时荧光定量PCR法检测MT1、MT3、ZnT1、ZnT3和ZIP1m RNA表达。结果与对照组相比,10μmol/L CORT处理HT-22细胞6h后,HT-22细胞内总锌含量显著下降,而先于CORT处理前加入糖皮质激素受体阻断剂RU486组总锌含量与对照组无明显变化。与对照组相比,CORT组HT-22细胞MT1、MT3、ZnT1、ZnT3、ZIP1 mRNA表达水平上升,而先于CORT处理前加入糖皮质激素受体阻断剂RU486组上述基因mRNA表达均无明显变化。结论 CORT可影响海马HT-22细胞锌稳态调节分子的表达,进而影响细胞锌稳态失衡。Objective To investigate the effects ofcorticosterone （CORT） on zinc level in hippocampal HT-22 cells in mice and its potential mechanisms. Methods HT-22 cells were cultured in DMEM and treated with 10μmol/L CORT for 6h. The zinc concentration in cells was measured by a flame atomic absorption spectrophotometer. Real time PCR was performed to determine the mRNA expression levels of MT1, MT3, ZnT1, ZnT3 and ZIP1 in CORT treated HT-22 cells. Results The zinc concentration in CORT-treated HT-22 cells decreased significantly （P〈0.05）, which was blocked by glucocorticoid receptor antagonist RU486. Treatment of 10μmol/L CORT for 6h significantly increased the MT1, MT3, ZIP1, ZnT1 and ZnT3 mRNA expression （P〈0.05）. Conclusion CORT could induce zinc dyshomeostasis in HT-22 cells via the regulations of some molecule involved in the uptake, excretion, and intracellular storage or trafficking of zinc.

关 键 词：皮质酮 海马 HI-22细胞 锌 锌稳态 

分 类 号：R363[医药卫生—病理学]