心理应激对肠易激综合征模型大鼠肠黏膜孤啡肽受体表达的影响  

Effect of psychological stress on expression levels of intestinal opioid-receptor-like-1 mRNA and protein in rats models of irritable bowel syndrome

作　　者：王鑫 巨伟 李武良 李萍 李鑫 

机构地区：[1]西安市第九医院消化内科,陕西西安710054

出　　处：《胃肠病学和肝病学杂志》2017年第5期514-517,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：2010年陕西省卫生厅科研基金

摘　　要：目的检测心理应激对肠易激综合征(irritable bowel syndrome,IBS)大鼠肠黏膜孤啡肽受体(ORL-1)mRNA和蛋白表达水平,初步探讨心理应激对IBS大鼠的影响。方法 80只SD雌性大鼠随机分为对照组和实验组,每组40只,实验组采用束缚应激的方法制作IBS动物模型,并对模型进行鉴定。实验0、1、2、3、4周时两组分别随机取出8只大鼠,在近端结肠(距回盲部2 cm)处取2块黏膜,采用荧光定量PCR法检测ORL-1 mRNA的相对表达量,同时用Western blotting的方法检测ORL-1蛋白,动态观察各组大鼠的表达变化。结果与对照组相比,实验组大鼠ORL-1 mRNA及蛋白表达水平均升高(P<0.05),应激1周时最高,2~4周逐渐下降。实验组与对照组差异有统计学意义(P<0.05)。结论慢性心理应激引起大鼠IBS的发生机制可能与其影响大鼠肠道肠黏膜孤啡肽受体(ORL-1)mRNA及蛋白表达量相关。Objective To investigate the effect of psychological stress on expression levels of opioid-receptor like-1 （ORL-1） mRNA and protein in irritable bowel syndrome （IBS） models rats. Methods Eighty Sprague-Dawley rats were randomly divided into control group （n = 40） and model group （n = 40）. IBS models were established by restrain stress method. Eight rats in each group were sacrificed at 0, 1, 2, 3, 4 weeks, respectively, and a partial colon tissue close to the ileocecal region about 2 cm in length was taken; and expression level of ORL1 mRNA was detected by reverse transcription-polymerase chain reaction （RT-PCR） , and expression level of ORL1 protein was detected by Western blotting. Results Compared with the control group, the expression levels of ORL1 mRNA and protein significantly were increased in model group at 1, 2, 3 and 4 weeks （P 〈 0.05）. The expression levels of ORL1 mRNA and protein at 1 week was the highest in model group, then gradually decreased at 2 - 4 weeks. Conclusion The effect of psychological stress on IBS in rats is possibly related to the up-regulation of expression levels of ORL1 mRNA and protein.

关 键 词：心理应激 肠易激综合征 孤啡肽受体 荧光定量PCR法 Western BLOTTING 

分 类 号：R574.4[医药卫生—消化系统]