3种植物生长调节剂对坛紫菜自由丝状体生长与光合色素含量的影响  

Effects of three plant growth regulators on the growth and photosynthetic pigment contents of free-living filaments of Pyropia haitanensis

作　　者：钟晨辉[1,2] 林琪[1,2] 张鹏[3] 黄瑞芳[1,2] 林向阳[1,2] 刘燕飞 

机构地区：[1]福建省水产研究所,厦门361013 [2]福建省海洋生物增养殖与高值化利用重点实验室,厦门361013 [3]浙江省海洋水产养殖研究所,温州325005 [4]福建省水产技术推广总站,福州350003

出　　处：《海洋渔业》2017年第2期216-224,共9页Marine Fisheries

基　　金：福建省种业创新与产业化工程项目(2014S1477-10);福建省自然科学基金(2014J01095);福建省海洋高新产业发展专项(闽海洋高新2014-19);福建省海洋与渔业结构调整专项;福建省海洋经济创新发展区域示范项目(2014FJPT01);厦门南方海洋研究中心资助项目(15GZY021NF04;14PZY017NF17);浙江省科技计划资助项目(2016F50033)

摘　　要：采用单因素分析法,比较研究了添加3种外源植物生长调节剂(吲哚乙酸IAA、2,4-二氯苯氧乙酸2,4-D、激动素KT)对野生型坛紫菜(Pyropia haitanensis)自由丝状体生长及光合色素含量的影响。结果表明:与对照组相比,低浓度的IAA(0.5~4.0 mg·L^(-1))和KT(0.5~2.0 mg·L^(-1))对藻体的特定生长率有显著影响,以2.0 mg·L^(-1)IAA和1.0 mg·L^(-1)KT对藻体的促生长效应最大,培养25 d时藻体鲜重增加最大,分别是对照组的1.90倍和1.52倍(P<0.05)。高浓度的IAA(6.0~10.0 mg·L^(-1))和KT(4.0~10.0 mg·L^(-1))却会抑制藻体生长(P<0.05)。培养25 d时,与对照组相比,2.0 mg·L^(-1)IAA和1.0 mg·L^(-1)KT均有利于叶绿素a(Chl a)和类胡萝卜素(Car)的合成,Chl a含量分别增加了23.7%和23.8%;Car含量分别增加了31.0%和28.6%(P<0.05)。然而,高浓度的IAA和KT(6.0、10.0 mg·L^(-1))不利于Chl a的合成,其处理浓度越高,Chl a含量下降越多。与对照组相比,较低浓度的IAA(1.0~2.0 mg·L^(-1))明显促进藻红蛋白(PE)和藻蓝蛋白(PC)合成,其中2.0 mg·L^(-1)IAA处理组PE、PC含量最高,分别增加了59.1%和45.7%(P<0.05)。较低浓度的KT(0.5~2.0 mg·L^(-1))均有利于PE、PC合成,其中0.5 mg·L^(-1)KT处理组的PE、PC含量增加最多,分别提高了48.4%和50.5%(P<0.05)。高浓度的IAA(6.0、10.0 mg·L^(-1))和KT(10.0 mg·L^(-1))则明显降低了PE、PC合成(P<0.05)。2,4-D对藻体的特定生长率和光合色素合成无显著影响(P>0.05)。The effects of three plant growth regulators, indole-3-acetic acid（ IAA）, 2, 4-dichlorophenoxyacetic acid（ 2,4-D） and kinetin（ KT）,with concentrations of 0. 0,0. 5,1. 0,2. 0,4. 0,6. 0and 10. 0 mg·L（-1）acting as the treatments in a single factorial comparative experiment,on the growth and photosynthetic pigment contents of free-living filaments in Pyropia haitanensis was studied. The results showed that compared to the control group low concentrations of IAA（ 0. 5 ~ 4. 0 mg·L（-1）） and KT（ 0. 5 ~ 2. 0 mg·L（-1）） had apparent influences on the specific growth rate of filaments,and the most obvious effects were observed in 2. 0 mg·L（-1）IAA and 1. 0 mg · L（-1）KT group,whose algal biomass were 0. 90 and 0. 52 time higher than those of the control group after 25 d culture,respectively（ P〈0. 05）. However,the high concentrations of IAA and KT（ 6. 0 ~ 10. 0 mg ·L（-1）） significantly inhibited the growth of filaments（ P〈0. 05）. After 25 d culture,compared to the control group,both 2. 0 mg · L（-1）IAA and 1. 0 mg · L（-1）KT significantly stimulated synthesis of Chlorophyll α（ Chl a） and Carotenoids（ Car）,and the contents of Chl a increased by 23. 7% and 23. 8%,respectively. While the contents of Car increased by 31. 0% and 28. 6%,respectively（ P〈0. 05）. Whereas,high concentrations of IAA and KT（ 6. 0 ~ 10. 0 mg·L（-1）） significantly reduced the contents of Chl a,presenting as the treated concentration higher,the Chl a synthesis less. In additional,low concentrations of IAA（ 1. 0 ~ 2. 0 mg·L（-1）） treatmeat significantly increased the contents of Phycoerythrin（ PE） and Phycocyanin（ PC）,which were highest in 2. 0 mg·L（-1）IAA group,and increased by 59. 1% and 45. 7%,respectively（ P〈0. 05）. Meanwhile,low concentrations of KT（ 0. 5 ~ 2. 0 mg ·L（-1）） resulted in massive synthesis of the PE and PC,which were the highest in 0. 5 mg·L（-1）KT group,and increased by 48. 4% and 50. 5%,respectively（ P�

关 键 词：坛紫菜 自由丝状体 植物生长调节剂 生长 光合色素 

分 类 号：S968.43[农业科学—水产养殖]