桑园土壤中高产纤维素酶菌株的筛选与鉴定  

Screening and Identification of High Cellulase-producing Bacteriafrom Soil at Mulberry Fields

作　　者：罗奉奉[1] 张昌伟[1] 莫亚玲 岑忠用[1] 

机构地区：[1]河池学院化学与生物工程学院,广西宜州546300

出　　处：《福建农业学报》2017年第4期425-430,共6页Fujian Journal of Agricultural Sciences

基　　金：广西高校微生物及植物资源开发利用重点实验室开放课题(2015HL008);河池学院重点科研课题(XJ2016ZD002)

摘　　要：为获得高产纤维素酶菌株,以桑园土壤为对象筛选纤维素酶产生菌。以羧甲基纤维素钠(CMC-Na)作唯一碳源,利用刚果红染色法从桑园土壤中筛选得到12株产纤维素酶菌株,其中菌株YZB46产酶效果最好,经酶学性质初步分析,YZB46在pH 6.0、40℃条件下发挥最佳内切葡聚糖酶活性,为17.08 U·mL^(-1),经传代,YZB46的产纤维素酶能力能稳定遗传。通过形态观察、革兰氏染色、生理生化特征及16SrDNA分析,发现菌株YZB46与芽孢杆菌属的Bacillus cereus同源性达99%,并将菌株YZB46鉴定为蜡样芽孢杆菌(Bacillus cereus)。High cellulase-producing strains of microorganisms were screened and identified from soil at mulberry fields.Using CMC-Na as the sole carbon source,12 target bacteria were isolated with Congo red dyeing.YZB46 was found to show the greatest cellulasesecretion among all.Preliminary analysis on thesecreted metabolitesindicated that the endo-cellulaseactivityreached 17.08 U·mL^(-1) at pH 6.0and 40 ℃,and the enzymedisplayed a desirable genetic stability.Based on the results of morphological observation,gram-staining,physiological and biochemical characterizations,as well as 16 SrDNA sequencing,the isolated YZB46 appeared to beclosely related to Bacillus cereus.

关 键 词：纤维素酶 筛选 鉴定 

分 类 号：Q939.9[生物学—微生物学]