基于2种衍生化方法对牛乳中寡糖的高效液相色谱-质谱联用分析  

Comparative Analysis of Oligosaccharides in Milk Based on Two Different Derivatization Methods by HPLC-Tandem Mass Spectrometry

作　　者：陈新新[1,2] 芦晶[2] 刘鹭[2] 逄晓阳[2] 张书文[2] 李诚[1] 吕加平[2] 

机构地区：[1]四川农业大学食品学院,四川雅安625014 [2]中国农业科学院农产品加工研究所,北京100193

出　　处：《食品科学》2017年第10期162-167,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目(1401514);“十二五”国家科技支撑计划项目(2013BAD18B10);公益性行业(农业)科研专项(201303085)

摘　　要：利用Sep-Pak C_(18)柱和石墨化碳柱对已脱脂和除去蛋白质的牛乳进行分离纯化,并采用1-苯基-3-甲基-5-唑啉酮(3-methyl-1-phenyl-2-pyrazolin-5-one,PMP)和2-氨基吖啶酮(2-aminoacridone,AMAC),2种衍生化试剂对其进行衍生化处理,通过高效液相色谱进行优化,AMAC衍生化的结果(约有67个峰)明显优于PMP衍生化的结果(约20个峰),最终确定AMAC为最佳衍生化试剂,并利用最终优化好的高效液相色谱条件,采用高效液相色谱-串联离子阱飞行时间质谱对牛乳中寡糖进行测定,最终测得7种乳寡糖并获得该7种乳寡糖的单糖组成。In the present study, Sep-Pak C18 and porous graphitized carbon column chromatographies were sequentiused to separate and purify oligosaccharides from defatted and deproteinated milk. After derivatization with phenymethyl-5-pyrazolone （PMP） or 2-aminoacridone （AMAC）, the oligosaccharides were analyzed by high-performance liqchromatography ion trap time of flight tandem mass spectrometry （HPLC-U-TOF-MS）. AMAC was a better derivatization agthan PMP, since they produced about 67 and 20 chromatographic peaks, respectively. Finally, seven lacto-oligosaccharides widentified under optimized HPLC conditions, and their monosaccharide compositions were determined.

关 键 词：牛乳寡糖 1-苯基-3-甲基-5-唑啉酮 2-氨基吖啶酮 高效液相色谱-串联离子阱飞行时间质谱 

分 类 号：O629.1[理学—有机化学]