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作 者:王奇惠 时永强[1] 祝宇[1] 张赛飞[1] 朱立力[1] 曲伟杰[1]
机构地区:[1]云南农业大学动物科学技术学院,云南昆明650201
出 处:《动物医学进展》2017年第5期77-80,共4页Progress In Veterinary Medicine
基 金:国家自然科学基金项目(31260629)
摘 要:对金黄色葡萄球菌小菌落突变株(small colony mutant strains Staphylococcus aureus,SASCVs)和CP000253.1基因组差异进行了研究,为后续研究金葡菌分泌抗药物相关物质能力提供数据支持。对一株金葡菌小菌落突变株进行全基因组测序,并与CP000253.1参考序列进行序列比较。结果显示,金葡菌SCVs的文库插入片段为350bp,测序读长为150bp,SCVs的原始数据量为551 Mb,SCVs的碱基含量分布在处理前后有所不同,通过重测序,存在单核苷酸多态性(SNP)的数量较大。金葡菌SCVs与CP000253.1参考序列之间存在明显的序列差异,该数据为研究金葡菌感染奶牛乳房炎提供了一定的序列依据。To study on the genome difference between the small colony mutant strains of Staphylococcus aureus (SASCVs) and the CP000253.1 for providing data to support subsequent research on the capacity of S.aureus bacteria secrete of drug related materials.We did a research on a whole genome sequencing of the small colony mutant strains of Staphylococcus aureus, and compared with those of sequence of CP000253.1 reference sequence.The results revealed that the library into fragments of the small colony mutant strains of Staphylococcus aureus is 350 bp.The sequencing read long is 150 bp.The amount of raw data of SCVs is 551 Mb. The base content of SCVs distribution is different before and after processing. By sequencing weight,the number of SNP is larger.There is an obvious sequence difference between the small colony mutant strains of Staphylococcus aureus and the CP000253.1 genome.The data provided the basis in a certain sequence for the study of dairy cow mastitis S.aureus infection.
关 键 词:金葡菌小菌落突变株 基因序列差异 全基因组测序 单核苷酸多态性
分 类 号:S852.611[农业科学—基础兽医学]
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