SerpinB5和β-catenin在原发性肝细胞癌中的表达及  

Experimental study of SerpinB5 and β-catenin in primary hepatocellular carcinoma

作　　者：孙海凤 郭亚焕 经里 黎明[3] 薛丽[5] 赵征 南克俊[4] 杨广宁 

机构地区：[1]陕西省肿瘤医院肿瘤内科,西安710061 [2]西安交通大学医学部 [3]西安交通大学第一附属医院心脏外科 [4]西安交通大学第一附属医院肿瘤内科 [5]西安交通大学第二附属医院检验科

出　　处：《中国医师进修杂志》2017年第5期385-389,共5页Chinese Journal of Postgraduates of Medicine

基　　金：陕西省自然科学基础研究计划项目（2014JM4183）

摘　　要：目的探讨SerpinB5和β-catenin在原发性肝细胞癌（HCC）发生和发展中的作用。方法采用免疫组织化学和实时定量反转录-聚合酶链反应（RT-PCR）的方法检测60例原发性HCC患者癌组织和癌旁组织中SerpinB5和β-catenin蛋白及mRNA的表达情况。结果癌组织中SerpinB5蛋白阳性表达率和SerpinB5 mRNA明显低于癌旁组织[25.0%（15/60）比63.3%（38/60）和1.12 ± 0.43比5.19 ± 0.39]，差异有统计学意义（P 〈 0.01）。癌组织中β-catenin蛋白阳性表达率和β-catenin mRNA明显高于癌旁组织[65.0%（39/60）比31.7%（19/60）和4.23 ± 0.25比1.19 ± 0.17]，差异有统计学意义（P 〈 0.01）。SerpinB5阳性表达降低与甲胎蛋白升高、肿瘤直径增大、分化程度差、TNM分期较晚、包膜侵犯及脉管癌栓有关（P〈0.01或〈0.05），β-catenin阳性表达增加与分化程度差、TNM分期较晚、包膜侵犯及脉管癌栓有关（P〈0.01或〈0.05）。相关性分析结果显示，SerpinB5与β-catenin的表达呈明显负相关（癌组织：r = -0.346, P = 0.001，癌旁组织：r= -0.258，P = 0.024）。结论SerpinB5和β-catenin的异常表达与原发性HCC的进展、恶性生物学行为密切相关，且两者在原发性HCC的发生和发展中存在协同作用。ObjectiveTo explore the role of the SerpinB5 and β-catenin in occurrence and development of the primary hepatocellular carcinoma （HCC）.MethodsThe expressions of SerpinB5 and β-catenin protein and mRNA in carcinoma tissues and paracancerous tissues of 60 patients with primary HCC were detected by immumohistochemistry and real-time quantitative reverse transcriptional polymerase chain reaction （RT-PCR） methods.ResultsThe positive expression rate of SerpinB5 protein and SerpinB5 mRNA in carcinoma tissues were significantly lower than those in paracancerous tissues： 25.0% （15/60） vs. 63.3% （38/60） and 1.12 ± 0.43 vs. 5.19 ± 0.39, and there were statistical differences（P〈0.01）. The positive expression rate of β-catenin protein and β-catenin mRNA in carcinoma tissues were significantly higher than those that in paracancerous tissues： 65.0% （39/60） vs. 31.7% （19/60） and 4.23 ± 0.25 vs. 1.19 ± 0.17, and there was statistical difference （P〈0.01）. Decreased SerpinB5 expression was associated with higher serumα-fetoprotein level, larger tumor size, poor differentiation, advanced TNM stage, capsule invasion and tumor thrombosis （P 〈 0.01 or 0.05）. Inecreased β-catenin expression was associated with poor differentiation, advanced TNM stage, capsule invasion and tumor thrombosis （P 〈 0.01 or 〈 0.05）. The correlation analysis result showed that SerpinB5 had negative correlation with β-catenin （carcinoma issues： r=-0.346, P = 0.001; paracancerous tissues： r =-0.258, P= 0.024）.ConclusionsThe abnormal expression of SerpinB5 and β-catenin may contribute to the progression and biologically malignant behavior of primary HCC, and SerpinB5 and β-catenin exists synergistic effect in the occurrence and development of primary HCC.

关 键 词：癌 肝细胞 SerpinB5 Β-CATENIN 

分 类 号：R735.7[医药卫生—肿瘤]