银杏内脂B微乳油剂对视网膜神经节细胞保护作用研究  

Protective Effect of Ginkgolide B Microemulsion Oil Agent on Retinal Ganglion Cells

作　　者：方敏[1] 胡慧玲[1] 郭梦翔 

机构地区：[1]深圳市眼科医院,深圳眼科学重点实验室,深圳市眼外伤治疗与干细胞定向分化公共服务平台,暨南大学附属深圳眼科医院,广东深圳518040 [2]广州市妇女儿童医疗中心,广东广州510060

出　　处：《辽宁中医药大学学报》2017年第5期38-41,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：广东省中医药局非资助项目(20152056)

摘　　要：目的:研究银杏内脂B微乳油剂对视网膜神经节细胞的保护作用,探讨其可能的作用机制,从而为其治疗青光眼等视网膜相关疾病提供理论依据。方法:选取出生后46 d的SD大鼠30只;随机抽取10只为A组(正常对照组);余下20只建立青光眼大鼠模型,按照SD大鼠体重1次腹腔注射60 mg/kg N-甲基-N-亚硝脲建立青光眼大鼠模型,并分为两组即B组(模型对照组)和C组(银杏内酯B治疗组),每组10只;A组不做任何处理,C组SD大鼠在实验前7 d每日腹腔注射银杏内酯B微乳油剂40 mg/kg;B组SD大鼠给等量生理盐水灌胃;实验1周、2周后分别行闪光视觉诱发电位及视网膜形态学分析测量中心视网膜外层厚度。结果:实验1周后,正常对照组、模型对照组、银杏内酯B治疗组的LP1和AP1分别为[(91±3)ms,(18±4)μV]、[(110±4)ms,(13±4)μV]、[(95±3)ms,(17±3)μV],银杏内酯B治疗组与正常对照组、模型对照组存在显著性差异,差异有统计学意义(P<0.05)。实验后2周,形态学检查结果表明正常对照组大鼠的中心视网膜外层厚度为(97.4±1.6)μm,模型对照组外视网膜厚度为(16.8±2.1)μm,而银杏内酯B治疗组外视网膜厚度为(24.2±2.3)μm,模型对照组与银杏内酯B治疗组均明显高于正常对照组,差异有统计学意义(P<0.05);而银杏内酯B治疗组显著高于模型对照组,差异有统计学意义(P<0.05)。结论:银杏内脂B微乳油剂对N-甲基-N-亚硝脲所致青光眼大鼠的视网膜神经细胞具有一定的保护作用,其可能的机制在于银杏内脂B通过调节青光眼大鼠的动作电位,同时修复损伤的视网膜,从而发挥视网膜保护作用。本研究对银杏内脂B微乳油剂视网膜神经节细胞的保护作用,并揭示了其作用机制,对银杏内脂B微乳油剂在青光眼等视网膜相关疾病的治疗提供了重要的理论基础。Objective： To research of ginkgolide B microemulsion oil agent on retinal ganglion cell protective effect of its possible mechanism, so as to provide a theoretical basis for the treatment of glaucoma and retinal diseases. Methods： 30 SD rats were selected at 46 days after birth; Random selected 10 for group A （ normal control group ）; the remaining 20 established glaucoma model in SD rat body weight intraperitoneal injection of 60 mg/kg N-methyl-N nitrosourea urea established glaucoma rat model, and divided into two groups：group B （model group ）and group C （gingkolide B treatment group ）, each group 10 rats ; A group did not do any treatment, group C of SD rat daily intraperitoneal injection of ginkgolide B microemulsion oil agent 40 mg/kg; B group SD rats were fed with the same amount of saline gavage; Experiment 1 week,2 weeks respectively, and the flash visual evoked potential and retinal morphology analysis and measurement center of the outer retina thickness. Results ： After 1 week of the experiment, the normal control group, model control group, ginkgolide B treatment group the Lpl and AP1 respectively[ （91±3）ms,（18±4） μV], [ （110-±4）ms,（13±4） μV], [ （95±3）ms,（ 17± 3 ）μ V], ginkgolide B treatment group and normal control group, model control group were significant difference, the difference was statistically significant （ P〈0.05 ）. The second week of the experiment, morphological examination results showed that normal control group rat central retinal thickness of the outer layer is （ 97.4± 1.6 ） μm, model control group, the retinal thickness was （ 16.8 ± 2.1 ）μm, and ginkgolide B treatment group retinal thickness for （ 24.2 ±2.3 ） μm, model control group and ginkgo inner ester B treatment group were significantly higher than those in normal control group, the difference was statistically significant （ P〈0.05 ）; and ginkgolide B treatment group was significantly higher than that of the model control group, the difference is s

关 键 词：银杏内酯B微乳油剂 青光眼 视网膜神经节细胞 闪光视觉诱发电位 视网膜厚度 

分 类 号：R775[医药卫生—眼科]