氨基隐性孔雀石绿抗体的制备及ELISA方法的建立  

Preparation of Antibody of Amino-Leucomalachite Green and Development of ELISA Method

作　　者：常超[1] 张佳艳[1] 程时杰[1] 伍金娥[1] 

机构地区：[1]武汉轻工大学食品科学与工程学院,武汉430023

出　　处：《湖北农业科学》2017年第7期1340-1344,共5页Hubei Agricultural Sciences

基　　金：武汉市科技计划项目(2013020501010180;2014020303010183)

摘　　要：以N,N-二甲基苯胺、对硝基苯甲醛为原料,合成改造了氨基隐性孔雀石绿半抗原,并通过紫外、红外、质谱鉴定。结果表明,氨基隐性孔雀石绿半抗原改造成功。采用重氮化法合成了氨基隐性孔雀石绿人工抗原,并通过紫外光谱进行鉴定,结果显示偶联蛋白成功。免疫大白兔制备了隐性孔雀石绿抗体,抗体效价达6.4×10~4。建立了隐性孔雀石绿ELISA方法,最低检测线为0.1μg/L。With N,N-dimethylanilin and p-nitrobenzaldehyde as raw materials,the hapten of amino-leucomalachite green （NH2-LMG） was synthesized and then was identified with ultraviolet,infrared and mass spectrum. The results showed that the artificial antigen of NH2-LMG was successfully synthesized by diazotization method and was identified by ultraviolet spectrum, which indicated the success of the coupling protein. The antibody of NH2-LMG was prepared with immune New Zealand white rabbits,and the antibody titer reached 6.4 ＆#215;104. The ELISA method was developed, with the limit of detection of 0.1 ug/L.

关 键 词：孔雀石绿 隐性孔雀石绿 抗体 ELISA 

分 类 号：O657.73[理学—分析化学]