Lack of association between integrin αvβ3 gene polymorphisms and hemorrhagic fever with renal syndrome in Han Chinese from Hubei, China  

作　　者：Xiao-Ping Chen Hai-Rong Xiong Ni Zhu Qing-Zhou Chen Hui Wang Chao-Jie Zhong Mei-Rong Wang Shuang Lu Fan Luo Wei Hou 

机构地区：[1]State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University

出　　处：《Virologica Sinica》2017年第1期73-79,共7页中国病毒学（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China (grant nos. 81101258, 81000734, and 81271819);a grant from Hubei Province Health and Family Planning Scientific Research Project (grant no. WJ2015MB113)

摘　　要：Hantaviruses belong to the family Bunyaviridae and cause hemorrhagic fever with renal syndrome （HFRS） in humans. 133 integrins, including αvβ3 and αⅡbβ3 integrins, act as receptors on endothelial cells and play key roles in cellular entry during the pathogenesis of hantaviruses. Previous study demonstrated that the polymorphisms of integrin αⅡbβ3 are associated with susceptibility to hantavirus infection and the disease severity of HFRS in Shaanxi Province of China, rather than in Finland. However, the polymorphisms of integrin av133 in patients with HFRS was incompletely understood. Here, we aimed to investigate the associations between polymorphisms in human integrin αvβ3 and HFRS in Han Chinese individuals. Ninety patients with HFRS and 101 healthy controls were enrolled in this study. Analysis of five single nucleotide polymorphism （SNP） sites （rs3768777 and rs3738919 on ITGAV; rs13306487, rs5921, and rs5918 on ITGB3） was performed by TaqMan SNP genotyping assays and bi-directional PCR allele-specific amplification method. No significant differences were observed between the HFRS group and controls regarding the genotype and allele frequency distributions of any of the five SNP sites, and no associations were found between ITGAV polymorphisms/genotypes and disease severity. In conclusion, our results implied that these five SNPs in the integrin αvβ3 gene were not associated with HFRS susceptibility or severity in Han Chinese individuals in Hubei Province.Hantaviruses belong to the family Bunyaviridae and cause hemorrhagic fever with renal syndrome(HFRS) in humans. β_3 integrins, including α_Vβ_3 and α_(Ⅱb)β_3 integrins, act as receptors on endothelial cells and play key roles in cellular entry during the pathogenesis of hantaviruses. Previous study demonstrated that the polymorphisms of integrin α_(Ⅱb)β_3are associated with susceptibility to hantavirus infection and the disease severity of HFRS in Shaanxi Province of China, rather than in Finland. However, the polymorphisms of integrin α_vβ_3 in patients with HFRS was incompletely understood. Here, we aimed to investigate the associations between polymorphisms in human integrin α_vβ_3 and HFRS in Han Chinese individuals. Ninety patients with HFRS and 101 healthy controls were enrolled in this study. Analysis of five single nucleotide polymorphism(SNP) sites(rs3768777 and rs3738919 on ITGAV; rs13306487, rs5921, and rs5918 on ITGB3) was performed by Taq Man SNP genotyping assays and bi-directional PCR allele-specific amplification method. No significant differences were observed between the HFRS group and controls regarding the genotype and allele frequency distributions of any of the five SNP sites, and no associations were found between ITGAV polymorphisms/genotypes and disease severity. In conclusion, our results implied that these five SNPs in the integrin α_vβ_3 gene were not associated with HFRS susceptibility or severity in Han Chinese individuals in Hubei Province.

关 键 词：hemorrhagic fever with renal syndrome （HFRS） POLYMORPHISMS HANTAVIRUS integrin αvβ3 

分 类 号：R512.8[医药卫生—内科学]