基于亚甲基蓝与DNA相互作用自铸成膜研究  

Preparation of Methylene Blue-Bound DNA Films by Self-Standing Casting Methods

作　　者：甘绮婷 陈琳琳[1] 朱耀婷 关海欣[1] 李红[1] 

机构地区：[1]华南师范大学化学与环境学院,广州510006

出　　处：《华南师范大学学报（自然科学版）》2017年第2期1-5,共5页Journal of South China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(21271075)

摘　　要：基于亚甲基蓝(MB)与DNA间的静电作用与嵌入作用,运用自铸膜法在载玻片上制备了蓝色的MB-DNA复合膜,并应用紫外-可见光谱法、荧光光谱法和扫描电镜等方法对复合膜进行了表征,研究了DNA-MB复合膜的成膜机制.结果表明,MB能嵌入诱导DNA的凝聚,形成均匀的MB-DNA复合膜,而膜中的DNA对MB起分散作用,使膜中多聚体MB分散成单体和二聚体的形式,显示出单体和二聚体的光吸收特性.该研究结果为DNA介导的功能光电器件的构筑提供了有力的支撑.A blue composite film is assembled on a glass substrate using a self-standing casting method based on the electrostatic attraction and intercalation of methylene blue （MB） with DNA. The resulting cast film is characterized by UV-visible absorption spectroscopy, fluorescence spectroscopy and scanning electron microscopy, and its casting mechanism mediated by DNA is further explored. The results suggest that MB can intercalate into DNA and simultane- ously induce the condensation of DNA to form a uniform MB-DNA composite film. The aggregated forms of MB can be disassociated into monomer or dimer forms based on the dispersion of DNA, revealed by their photoabsorption proper- ties. This study provides a powerful basis for constructing DNA-mediated optoelectronic functional devices.

关 键 词：亚甲基蓝 DNA 复合膜 自铸膜法 

分 类 号：O657.3[理学—分析化学]