p62/SQSTM1在鼻咽癌中的表达  

Expression of p62/SQSTM1 in nasopharyngeal carcinoma

作　　者：向满林 吴琼[1] 王堃[1] 梁运来 谢旭琳 易斌[1] 

机构地区：[1]中南大学湘雅医院检验科,长沙410008

出　　处：《临床与病理杂志》2017年第4期802-807,共6页Journal of Clinical and Pathological Research

基　　金：2016年中南大学硕士生自主探索创新项目(2016zzts529)~~

摘　　要：目的:研究p62在鼻咽癌(nasopharyngeal carcinoma,NPC)细胞、组织中的表达情况及其自身抗体在外周血中的水平,探究p62与NPC发生、转移的关系及其在临床中的应用价值。方法:采用Western印迹法和免疫组织化学染色法分别检测NPC细胞株NP69,6-10B和5-8F和NPC患者组织中的p62表达;ELISA检测患者血清中抗p62抗体浓度,并分析其与临床病理特征的关系。结果:p62在NP69,6-10B和5-8F细胞中的表达水平依次升高(P<0.05);NPC组织中p62高表达,其水平与患者有无转移(χ~2=9.332,P=0.002)有关。NPC患者血清中抗p62水平较慢性鼻咽炎组明显升高(t=–4.653,P<0.001),且抗p62抗体水平与患者是否转移有关(t=14.255,P=0.016);血清抗p62抗体筛查NPC的最佳临界值为51.82μg/L,判别是否发生转移的临界值为62.03μg/L。结论:p62高表达与NPC发生及转移密切相关,组织中p62蛋白和血清中抗p62抗体可能作为NPC病情监测和判断预后的新指标。Objective： To study the expression of p62 in nasopharyngeal carcinoma （NPC） cell lines and tissues, and explore the serum concentration of anti-p62 in NPC patients, and investigate the correlation between p62 and NPC occurrence and metastasis, and assess its clinical value. Methods： The expression of protein p62 in NPC cell lines （NP69, 6-10B and 5-8F） was detected by Western blot. Immunohistochemistry staining method was used to test the expression of p62 in NPC tissue specimens. Serum anti-p62 antibody level was determined by enzymeplinked immunosorbent assa）5 then analyzing its relationship with clinical pathological features. Results： The expression of p62 in NP69, 6-10B and 5-8F cells was increased successively （P〈0.05）; the expression of p62 was increased in nasopharyngeal carcinoma tissues, which was closely correlated with metastasis （χ2=9.332, P=0.002）. Compared with chronic rhinopharyngitis group, the serum level of anti-p62 in NPC patients was dramatically increased （t=-4.653, P〈0.001） and correlated with metastasis （t=14.255, P=0.016）; the optimal value of serum anti-p62 screening for NPC is 51.82 μg/L, and the critical value of anti-p62 judging NPC patients whether metastasis or not is 62.03 μg/L. Conclusion： High NPC, p62 protein in tissues and serum prognosis of NPC patients. expression of p62 is closely related to anti-p62 may be used as new indicators the occurrence and metastasis of for monitoring the conditions and

关 键 词：抗p62抗体 鼻咽癌 发生 转移 

分 类 号：R739.63[医药卫生—肿瘤]