桑葚的提取及其自由基清除活性研究  

Extraction and Free Radical Scavenging Activities of Mulberries

作　　者：刘茜[1] 唐洁[2] 

机构地区：[1]梧州学院化学工程与资源再利用学院,广西梧州543002 [2]桂林师范高等专科学校化学与工程技术系,广西桂林541001

出　　处：《食品研究与开发》2017年第3期181-183,共3页Food Research and Development

基　　金：广西高校中青年教师基础能力提升项目(KY2016YB441);梧州学院重点科研项目(2014B011)

摘　　要：在超声波辅助下,分别以无水乙醇、丙酮、蒸馏水为提取溶剂,对桑葚果实进行提取,浓缩后分别得无水乙醇提取物AE,丙酮提取物EE,蒸馏水提取物WE,通过测定提取物对DPPH·、ABTS^+·、·OH的清除作用评价桑葚的抗氧化活性。提取物AE、EE、WE对DPPH·的IC_(50)分别为0.173、0.18、0.19 mg/m L;对ABTS^+·的IC_(50)分别为0.044、0.052、0.06 mg/m L;对·OH的IC_(50)分别为0.67、0.83、1.02 mg/m L。提取物清除DPPH·、ABTS^+·、·OH的IC_(50)值均远远小于10 mg/m L,表明桑葚提取物具有良好的自由基清除活性。试验表明溶剂对提取物清除自由基的活性有一定影响。Under ultrasound, mulberries were extracted with ethanol, acetone and water solvents to offer ethanol extract （AE）, acetone extract （EE） and water extract （WE）, respectively. Their free radical scavenging activities were respectively evaluated against DPPH, ABTS^＋ and hydroxyl radicals. The IC50 values of WE, EE and AE against DPPH· and ABTS^＋· were found to be 0.173, 0.18,0.19 mg/mL and 0.044, 0.052, 0.06 mg/mL, respectively, while that of WE, EE and AE against OH^-· free radical were 0.67, 0.83, 1.02 mg/mL. The results showed that all IC50 of AE, EE and WE were much lower than the standard value 10 mg/mL in these three assays, suggesting all these extracts displayed good radical scavenging activities. In addition, the extract solvents had important effect on their radical scavenging activities.

关 键 词：桑葚 DPPH· ABTS^+· ·OH 自由基清除活性 

分 类 号：R284.2[医药卫生—中药学]