固定化纤维素酶及其对棉纤维的作用  

Immobilized Cellulase and Its Effect on Cotton Fibers

作　　者：郭陈岑 张宇[1] 张永奎[1] 谢通慧[1] 杨坤[1] 

机构地区：[1]四川大学化工学院,四川成都610065

出　　处：《纺织科技进展》2017年第5期1-4,共4页Progress in Textile Science & Technology

基　　金：四川大学大学生创新训练项目(201610611654)

摘　　要：为实现纤维素酶处理棉织物后重复使用,以pH敏感型树脂——聚丙烯酸树脂Ⅳ为载体,吸附固定纤维素酶。结果表明,纤维素酶成功固定于载体上,酸性条件下溶解,中性条件下析出回收,其酶活固定率与4次酶活回收率分别为45.5%和81.4%。与游离纤维素酶相比,固定化纤维素酶热稳定性增强,在酶精练过程中,其催化速率更为稳定可控。表征发现经固定化纤维素酶处理后棉纤维结晶度增大,吸水性、可染性增强,纤维卷曲减少,对纤维损伤程度较小。Polyacrylic resin IV of pH sensitive resin was used as carrier for immobilization of cellulase to ensure biotreatment of cellulose. The results showed that cellulase was immobilized on resin. It was dissolved under acid condition and precipitated under neu- tral condition. Activity fixed rate and recovery rate were 45.5%and 81.4%, respectively. Compared with free cellulase, immobilized cellulase had higher thermal stability. In scouring process, immobilized cellulase remained stable catalytic rate. The crystallinaty in- dex, water adsorption and dyeability of cotton were all increased and fiber crimp was decreased. Cotton treated with immobilized cellu- lase showed less damage.

关 键 词：纤维素酶 pH敏感型树脂 棉纤维 酶精练 

分 类 号：Q814[生物学—生物工程] TS192.22[轻工技术与工程—纺织化学与染整工程]