亚低温通过下调分化抑制因子2保护脑缺血再灌注损伤大鼠  

Mild hypothermia protects cerebral ischemia-reperfusion injury in rats by downregulating inhibitor of differentiation 2

作　　者：任力[1] 邢进[1] 魏子龙[1] 王之涵[1] 赵亮[1] 邱永明[2] 林盈盈[2] 

机构地区：[1]上海市浦东医院复旦大学附属浦东医院神经外科,201399 [2]上海交通大学附属仁济医院神经外科,200127

出　　处：《国际脑血管病杂志》2017年第3期223-227,共5页International Journal of Cerebrovascular Diseases

基　　金：上海市卫生和计划生育委员会中医药科研基金（2014JP025A）;上海市卫生和计划生育委员会面上项目（201540412）;浦东新区卫生系统重点专科建设项目（PWZz2013-18）

摘　　要：目的探讨亚低温对脑缺血再灌注大鼠的保护作用以及对分化抑制因子2（inhibitor of differentiation 2, Id2）蛋白表达的影响。方法72只成年雄性大鼠随机分为假手术组、常温组和亚低温组。应用线栓法制备大脑中动脉闭塞模型，亚低温组给予低温处理[肛温（33±1）℃，鼓膜温度（31±1）℃]。分别在缺血再灌注6、12、24和72 h时采用改良神经功能损伤严重程度评分（modified Neurological Severity Score, mNSS）评价神经功能缺损，氯化三苯基四氮唑检测梗死体积，蛋白质印迹法检测缺血皮质Id2表达水平。结果缺血再灌注6、12、24和72 h时，亚低温组mNSS评分均显著高于常温组，梗死体积均显著小于常温组（P均〈0.001）。蛋白质印迹分析显示，亚低温组脑缺血再灌注6、12、24和72 h时缺血皮质Id2蛋白表达水平显著低于常温组（P均〈0.05）。结论亚低温能减轻脑缺血再灌注后神经功能缺损和缩小梗死体积，其机制可能与下调Id2蛋白表达水平有关。Objective To investigate the protective effect of mild hypothermia on cerebral ischemiareperfusion injury in rats and the effect of mild hypothermia on the expression of inhibitor of differentiation 2 （Id2） protein. Methods A total of 72 adult male rats were randomly divided into a sham operation group, a normothermia group, and a mild hypothermia group. A model of middle cerebral artery occlusion was induced by a suture method. The mild hypothermia group was treated with low temperature （anal temperature 33 ± 1 ℃, tympanic membrane temperature 31 ± 1 ℃）. Modified Neurological Severity Score （mNSS） was used to evaluate neurological deficits, triphenyltetrazolium chloride staining was used to detect infarct volume, and Western blot was used to detect the Id2 expression in the ischemic cortex at ischemiareperfusion 6, 12, 24, and 72 h, respectively. Results The mNSS scores in the mild hypothermia group were significantly lower than those in the normothermia group, the infarct volumes were significantly smaller than those in the normothermia group at ischemia-reperfusion 6, 12, 24, and 72 h （all P 〈0. 001）. Western blot analysis showed that the Id2 expressions in the ischemic cortex in the mild hypothermia group were significantly lower than those in the normothermia group at ischemia-reperfusion 6, 12, 24, and 72 h （all P 〈 0. 05）. Conclusions Mild hypothermia can decrease neurological deficits and reduce infarct volume after cerebral ischemia-reperfusion, its mechanism may be associated with the down-regulation of the Id2 expression.

关 键 词：脑缺血 再灌注损伤 低温 人工 分化蛋白抑制剂2 细胞凋亡 疾病模型 动物 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]