木姜子乙醇提取物对肝癌细胞侵袭和迁移能力的影响及机制初探  

Effects and mechanism of ethanol extract of Litsea pungens on migration and invasion of hepatocellular carcinoma cells

作　　者：罗耀玲[1] 黄铀新[2] 杨建琼[1] 张敏鸿[1] 

机构地区：[1]赣南医学院第一附属医院临床医学研究中心,江西赣州341000 [2]赣南医学院第一附属医院核医学科,江西赣州341000

出　　处：《中国医院药学杂志》2017年第9期839-842,共4页Chinese Journal of Hospital Pharmacy

基　　金：江西省卫计委中医药科研课题(编号:2015A031)

摘　　要：目的:探讨木姜子乙醇提取物(Litsea pungens ethanol extract,LPEE)对肝癌细胞侵袭转移能力的影响及其相关机制。方法:MTT法测定LPEE对肝癌细胞株HepG2和QGY-7703的IC50;transwell迁移实验和matrigel侵袭实验检测LPEE对HepG2和QGY-7703细胞迁移和侵袭能力的影响;qRT-PCR和Western blot检测LPEE对各组细胞中E-cadherin和VEGF基因和蛋白水平的表达变化。结果:LPEE可显著抑制HepG2和QGY-7703细胞穿过transwell小室和matrigel基质胶的细胞数(P<0.05);50和100μg·mL^(-1) LPEE作用组与0组相比,可明显降低VEGF mRNA和蛋白水平的表达(P<0.05),而促进E-cadherin mRNA和蛋白水平的表达(P<0.05)。结论:LPEE可抑制肝癌细胞的侵袭和迁移能力,其机制可能与抑制VEGF表达和促进E-cadherin表达有关。OBJECTIVE To evaluate the effects and mechanism of ethanol extract of Litsea pungens（LPEE）on migration and invasion of hepatocellular carcinoma cells.METHODS IC50 values of LPEE on HepG2 and QGY-7703 were determined by MTT.The migratory and invasive abilities of the HepG2 and QGY-7703 cells were measured by Transwell chamber and Matrigel assays.The mRNA and protein expression of E-cadherin and VEGF were determined in HepG2 and QGY-7703 cells treated with LPEE by qRT-PCR and Western blot.RESULTS LPEE significantly suppressed the migration and invasion of tumor cells into the basement membranes（P〈0.05）.Compared with0 group,the groups of 50μg·mL^-1 and 100μg·mL^-1 LPEE significantly reduced the expression of mRNA and protein of VEGF（P〈0.05）,and promoted the expression of E-cadherin（P〈0.05）.CONCLUSION LPEE can reduce migratory and invasive abilities of hepatocellular carcinoma cells,which may be related to the inhibition of VEGF expression and the promotion of E-cadherin expression.

关 键 词：木姜子 HEPG2 QGY-7703 侵袭转移 

分 类 号：R285.5[医药卫生—中药学]