循环miR-4731-3p在肺癌患者血浆中的表达及临床意义  

Expression and clinical significance of miR-4731-3p in plasma of patients with lung cancer

作　　者：吕秀[1,2] 雷明盛 贾凤玲[2] 李赛[1] 张楚红 胡甜[1] 贺莎[1] 严汉兴 胡云[2] 陈荣如[1] 谭贻兮 文美玲[1] 左建宏[1,2] 

机构地区：[1]南华大学附属南华医院肿瘤内科,湖南衡阳421001 [2]南华大学医学院肿瘤研究所 [3]张家界市人民医院呼吸内科

出　　处：《中南医学科学杂志》2017年第3期255-258,共4页Medical Science Journal of Central South China

基　　金：国家自然科学基金(81272960);湖南省科技厅项目(2013WK2010);人社部重点项目[2016]176号;省卫计委项目[B2014-171];张家界市科技局项目[2015GK1128]

摘　　要：目的探讨microRNA-4731-3p(miR-4731-3p)在肺癌血浆中的表达及其临床意义。方法采用实时荧光定量PCR(qRT-PCR)方法检测40例肺癌患者和40例健康人(作为对照)血浆miR-4731-3p的表达水平,分析其与临床病理参数和预后的相关性。绘制受试者工作特征曲线(ROC),评价血浆miR-4731-3p表达水平对原发性肺癌的诊断价值。结果肺癌血浆中miR-4731-3p的表达水平(0.11±0.05)显著高于健康人(0.05±0.03)(P<0.001);miR-4731-3p表达水平与癌胚抗原(CEA)正相关(P<0.05),与患者性别、年龄、吸烟、病理类型、肿瘤大小、临床分期、远处转移均无明显相关性(P>0.05)。血浆miR-4731-3p在ROC曲线下面积(AUC)为0.835(95%CI=0.750~0.921)。当miR-4731-3p临界值取0.048时,对肺癌诊断的灵敏度为0.950,特异度为0.575。KaplanMeier分析显示肺癌血浆中miR-4731-3p低表达者生存时间明显高于高表达组(P<0.05)。结论血浆miR-4731-3p在肺癌血浆中表达上调,可作为肺癌诊断及预后判断的潜在分子标志物。Objective To investigate the expression of microRNA-4731-3p（ miR-4731-3p） in the plasma of patients with lung cancer and to determine the correlation between miR-4731-3p level and clinical parameters. Methods Quantitative real-time PCR（ qRT-PCR） was used to examine the plasma level of miR-4731-3p in 40 patients with lung cancer and 40 healthy persons（ control）.The correlation between the plasma miR-4731-3p level and clinicopathological features and prognosis were investigated.Receiver operating characteristic（ ROC） curve were drawn to evaluate the diagnosis value of miR-4731-3p expression in plasma for lung cancer. Results The plasma miR-4731-3p was significantly up-regulated in patients with lung cancer as compared with healthy subjects. The expression of miR-4731-3p was associated with carcino-embryonic antigen（ CEA）（ P〈0.05）,while it was not associated with gender,age,smoking,pathology type,tumor size,clinical stage and distant metastasis（ P〉0.05）.The area under the ROC curve（ AUC） according to the miR-4731-3p expression in plasma was 0.835（ 95% CI = 0.750 ~ 0.921）,when the critical value was 0.048.The sensitivity for lung cancer diagnosis and specific degrees were 0.950 and 0.575 respectively.The expression of miR-4731-3p was correlated with overall survival of lung cancer（ P〈0.05）. Conclusion The expression of miR-4731-3p is up-regulated in tumor,and it may be a potential biomarker for the diagnosis and prognosis evaluation of lung cancer.

关 键 词：肺癌 微小RNA-4731-3p 诊断 预后 

分 类 号：R734.2[医药卫生—肿瘤]