热性中药成分辣椒碱调节小鼠UCP1的分子机制研究  

Study on molecular mechanism of the hot-herb component capsaicin based on UCP1 regulation

作　　者：孟晶[1] 戴逸飞[1] 周炜炜[1] 李倩[1] 周海玉[1] 姬海南 戴丽[1] 王朋倩[1] 霍海如[1] 隋峰[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国药理学通报》2017年第3期338-342,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金面上项目(No 81274112;81373986;81473372);国家自然科学基金青年项目(No 8143125;81403322);北京市自然科学基金(No 7152016)

摘　　要：目的为进一步揭示热性中药的分子机制、诠释中药寒热药性理论的科学内涵,同时对能量代谢开关解偶联蛋白亚型1(UCP1)的表达调控作用进行研究。方法采用热偶技术对小鼠的核心温度和尾部皮肤温度进行了给药后的实时监测,温度的采集分析采用了热偶检测技术,UCP1的表达量分别采用了荧光定量PCR和Western blot的技术。结果辣椒碱给药后小鼠机体中心温度先降低后上升,尾部皮肤温度以升高为主,与空白对照组相比差异有显著性(P<0.05或P<0.01);UCP1的基因和蛋白的表达量均明显上调(P<0.05或P<0.01)。结论上调UCP1的表达进而提高机体的能量代谢可能是辣椒碱作为辣椒的热性成分表征热性属性的生物分子机制之一。Aim Tofurtheruncoverthemolecular mechanism of hot herbs and thus to decode the scientif-ic significance of herb nature theory,real-time check on the core body temperature and tail skin temperature of the capsaicin-administrated mice was conducted and the expression of UCP1 at the mRNA and protein levels wasalsoperformed.Methods Thetemperaturesof the mice were registered by the heat electronic couple technique,and the expression of UCP1 was assayed by the methods real-time PCR and Western blot respec-tively.Results Thecorebodytemperatureofthe mice after administration of capsaicin decreased andthen increased, while the tail skin temperature in-creased rapidly upon treatment with capsaicin （P 〈0. 05 or P〈0. 01 ）;both the mRNA and protein ex-pression levels of UCP1 were up-regulated（P〈0. 01）. Conclusion Promotiononenergymetabolismthough up-regulation of UCP1 may be one of the biologically molecular mechanisms for the capsaicin to demonstrate its characteristics in terms of cold-hot natures of Chi-nese herbs.

关 键 词：热性中药 药性理论 辣椒碱 能量代谢 分子机制 解偶联蛋白 

分 类 号：R-332[医药卫生] R284.1