CJ016对人肺癌裸鼠移植瘤的抑制活性研究  

Study of CJ016 in treatment of lung cancer

作　　者：甘萍[1] 谭永红[1] 金伟华[1] 王玲洁[1] 于波涛[1] 

机构地区：[1]成都军区总医院药剂科,四川成都610083

出　　处：《中国药理学通报》2017年第3期384-388,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年基金资助项目(No 81402493)

摘　　要：目的研究CJ016对人肺癌模型的治疗效果及其作用机制。方法建立人肺腺癌细胞A549裸鼠移植瘤模型,考察CJ016体内抑制肿瘤生长效果;同时将肿瘤进行免疫组化检测,考察其对肿瘤内CD31和细胞凋亡的影响。结果CJ016对A549细胞增殖有明显抑制作用,其IC_(50)值为34.22nmol·L^(-1);动物实验表明其具有较好的肿瘤抑制效果,抑瘤率、T/C%值分别为70.08%和27.75%(20 mg·kg^(-1));同时,CJ016能降低肿瘤细胞内CD31的表达,促进肿瘤细胞凋亡。结论 CJ016能明显抑制A549细胞荷瘤小鼠肿瘤生长,其可能的作用机制是减少血管生成和诱导肿瘤细胞凋亡。Aim Tostudythetherapeuticeffectof CJ016 on human lung cancer model and the mecha-nism.Methods Anexperimentalhumanlungadeno-carcinoma model of A549 was set up to investigate the anti-tumor effect of CJ016,while the effect of angio-genesis and apoptosis in tumor were detected.Results In vitro,the cell proliferation was inhibited signifi-cantly by CJ016,and the value of IC50 was 34. 22 nmol ·L-1 .In vivo,the tumor inhibition rate and T/C%value were 70. 08%and 27. 75%,respectively,at the dose of 20 mg·kg-1 .Meanwhile,CJ016 could reduce the expression of CD31 and promote the apoptosis of tumorcells.Conclusion CJ016caninhibitthegrowth of A549 cells,and the possible mechanism may be re-lated to the reduction of angiogenesis and inducing tumor cell apoptosis.

关 键 词：肺癌 作用机制 CJ016 A549 IC(50) CD31 细胞凋亡 

分 类 号：R-332[医药卫生] R329.25