氧化槐定碱对福尔马林致痛小鼠大脑GAT-1表达的影响  

Analgesic effect of oxysophoridine and its effect on brain GAT-1 in mice

作　　者：高进贤[1] 张秀娟[2] 余建强[3] 蒋袁絮[3] 

机构地区：[1]甘肃省人民医院药剂科,甘肃兰州730000 [2]甘肃省人民医院质控科,甘肃兰州730000 [3]宁夏医科大学药学院,宁夏银川750004

出　　处：《中国药理学通报》2017年第3期407-411,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30860372)

摘　　要：目的观察小鼠大脑GABA转运体1(GABA transporter-1,GAT-1)的变化对氧化槐定碱(oxysophoridine,OSR)镇痛作用的影响。方法采用福尔马林舔足法观察小鼠静脉(intravenous injection,iv)给药后OSR的镇痛效果,免疫组织化学方法(SABC法)观察OSR对小鼠大脑皮层和丘脑GAT-1免疫阳性细胞的影响,Real time RT-PCR方法检测OSR对福尔马林致痛小鼠大脑GAT-1 mRNA表达的影响。结果 iv OSR 500、250、125 mg·kg^(-1)可延长福尔马林所致小鼠的舔足潜伏期(P<0.05,P<0.01);腹腔注射(intraperitoneal,ip)OSR 500 mg·kg^(-1)可使小鼠丘脑和大脑皮层的GAT-1免疫阳性细胞表达明显减少(P<0.01),对小鼠大脑的GAT-1mRNA的表达也有明显的抑制作用(P<0.05)。结论 OSR有明显的镇痛作用,其镇痛作用机制与中枢GAT-1表达的下调相关。Aim Tostudytheanalgesiceffectofoxyso-phoridine （OSR）on GABA transporter-1 （GAT-1 ）mR-NA expression and its influence on GAT-1 expression inmice.Methods Formalintestwasusedtodetectthe analgesic effect of OSR（iv）.Immunohistochemis-try was taken to inspect the expression of GAT-1 in cerebral cortex and thalamus in mouse brain. The quantitative real-time PCR method was used to inspect the influence of OSR on GAT-1 mRNA expression of braininmice.Results OSR（500,250,125mg· kg-1 ,iv ） could significantly increase the foot-licking latency.OSR（500 mg·kg-1,ip）could significantly decrease the number of GAT-1 immuopositive cells incerebral cortex and thalamus in mouse brain,and re-duce GAT-1 mRNA expression in brain（P〈0. 01,P〈0.05）intheformalintest.Conclusion OSRhasa significant analgesic effect,and its analgesic mecha-nism is related to the GAT-1 expression in mouse brain.

关 键 词：氧化槐定碱 福尔马林 镇痛 GAT-1 GABA 大脑 

分 类 号：R-332[医药卫生] R284.1