氧糖剥夺再灌注引起的SH-SY5Y细胞凋亡与NF-κBp65和COX-2蛋白表达的关系  

The relationship between the apoptosis of SH-SY5Y cells and NF-κBp65,COX-2 after oxygen-glucose deprivation/reperfusion

作　　者：黄赟[1] 董艳军[2] 肖雁[1] 官志忠[1] 

机构地区：[1]贵州医科大学分子生物学重点实验室贵州医科大学地方病与少数民族疾病教育部重点实验室,贵州贵阳550004 [2]贵州省人民医院病理科,贵州贵阳550004

出　　处：《中风与神经疾病杂志》2017年第2期119-121,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金(No.81160149);贵州省科技厅基金项目[黔科合J字(2014)2012]

摘　　要：目的探讨氧糖剥夺再灌注(oxygen-glucose deprivation/reperfusion,OGD/R)后SH-SY5Y细胞凋亡情况及NF-κB信号通路NF-κBp65、COX-2蛋白的表达。方法将SH-SY5Y细胞分为正常对照组、氧糖剥夺4 h/再灌注24 h组(OGD/R)组。利用流式细胞术分别检测各组细胞凋亡率,蛋白印记法(Western blot)检测p65和COX-2的蛋白表达情况。结果与正常对照组比较,OGD/R组细胞凋亡率明显增高(P<0.05),p65和COX-2的蛋白表达也明显增高(P<0.05)。结论氧糖剥夺4 h/再灌注24 h能够促进细胞凋亡,其机制可能是通过NF-κBp65对COX-2调控的信号通路来实现。Objective To explore the relationship between the apoptosis of SH-SY5Y cells and NF-KBp65 , COX-2 after oxygen-glucose deprivation/reperfusion （OGD/R）. Methods Cells were divided into normal control group and oxygen glucose deprivation 4 h/reperfusion 24 h groups. The cell apoptosis rate was analyzed using flowcytometry, and Western blot assay was used to evaluate the expression of p65 and COX-2 proteins in control group and OGD/R group. Results Compared with the normal control group,the apoptosis rate of OGD/R group was significantly higher （P 〈 0 .0 5 ） ,and the protein expres-sion of p65 and COX-2 was also significantly increased （P 〈 0 .0 5 ） . Conclusion Oxygen glucose deprivation 4 h/reperfusion 24 h can induced apoptosis,which may be through the signaling pathway that NF-kB p65 regulates COX-2 to achieve.

关 键 词：氧糖剥夺再灌注 细胞凋亡 NF-κBp6 COX-2 

分 类 号：R743.3[医药卫生—神经病学与精神病学]