沉默信息调节因子7和p53在胃癌组织的表达及临床意义  

Expression of silent information regulator 7 and p53 in gastric cancer and clinical significance

作　　者：林海鹏[1] 卢晓明[1] 王莉霞[2] 吕清[1] 牛彦锋[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胃肠外科,武汉430022 [2]华中科技大学同济医学院附属协和医院放射科,武汉430022

出　　处：《中华实验外科杂志》2017年第5期850-852,共3页Chinese Journal of Experimental Surgery

基　　金：武汉市科学技术计划项目（2014062801011269）;湖北省卫生和计划生育委员会科研项目（WJ2015MB075）;华中科技大学自主创新研究基金（0118530321）

摘　　要：目的探讨沉默信息调节因子7（Sirt7）和p53在胃癌组织中的表达及其与胃癌临床病理特征的关系。方法应用免疫组织化学方法检测60例胃癌组织及癌旁组织中Sirt7和p53的表达，并随机选取20例胃癌及癌旁组织蛋白行Western blot检测，分别检测Sirt7和p53的表达水平。结果胃癌组织中Sirt7和p53的阳性率（63.33%、66.67%）明显高于癌旁正常组织的阳性率（21.67%、0.00%），差异有统计学意义（P=0.024, P=0.000）。Sirt7和p53在胃癌组织中的表达与患者年龄、性别无明显相关（χ2=0.057，P=0.811；χ2=0.028，P=0.866及χ2=0.564，P=0.453；χ2=0.566，P=0.566），而与胃癌肿瘤大小、淋巴结转移、浸润深度、胃癌TNM分期明显相关（χ2=6.007，P=0.014；χ2=7.578，P=0.006；χ2=8.403，P=0.004；χ2=15.083，P=0.002及χ2=6.652，P=0.010；χ2=4.431，P=0.035；χ2=4.660，P=0.031；χ2=9.947，P=0.019）。Western blot检测显示20例胃癌患者Sirt7和p53在胃癌组织的表达水平明显高于癌旁组织，差异有统计学意义（P=0.017）。配对资料相关性分析显示Sirt7和p53在胃癌组织中表达呈正相关（r=0.324，P=0.008）。结论Sirt7和p53在胃癌组织中高表达，且与胃癌肿瘤大小、淋巴结转移、浸润深度、胃癌TNM分期密切相关，表明Sirt7和p53可能参与胃癌的发病过程。ObjectiveTo study the expression level of silent information regulator 7 （Sirt7） and p53 in the gastric cancer, and their correlation with the gastric cancer.MethodsThe expression of Sirt7 and p53 protein was detected by immunohistochemistry in 60 cases of gastric carcinomas and para-carcinoma tissues. Western blotting was used to detect the protein expression of Sirt 7 and p53 in randomly chosen 20 cases of gastric carcinoma and adjacent tissues.ResultsThe positive rate of Sirt7 and p53 expression in gastric cancer （63.33% and 66.67%） was significantly higher than in the adjacent tissues （21.67% and 0.00%, P=0.024）. The expression of Sirt7 and p53 was not correlated to age and gender （χ2=0.057, P=0.811; χ2=0.028, P=0.866 and χ2=0.564, P=0.453; χ2=0.566, P=0.566）, but to the size of tumor, lymph node metastasis, depth of invasion and TNM staging （χ2=6.007, P=0.014; χ2=7.578, P=0.006; χ2=8.403, P=0.004; χ2=15.083, P=0.002 and χ2=6.652, P=0.010; χ2=4.431, P=0.035; χ2=4.660, P=0.031; χ2=9.947, P=0.019）. Western blotting showed that the expression of Sirt7 and p53 in gastric cancer was higher than in the normal tissues （P=0.017）. Correlation analysis showed that Sirt7 and p53 in gastric carcinoma were positively correlated （r=0.324, P=0.008）.ConclusionThe expression of Sirt7 and p53 in gastric cancer is higher than in adjacent tissues, and is correlated with the size of tumor, lymph node metastasis, depth of invasion and TNM staging, suggesting their roles in the pathogenesis of gastric cancer.

关 键 词：沉默信息调节因子7 P53 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]