色素上皮源性因子通过血管内皮生长因子抑制非小细胞肺癌的发生发展  

PEDF Inhibits Non-small Cell Lung Cancer Proliferation by Down-regulating VEGF Expression

作　　者：周立霞 吴月明 李娜 

机构地区：[1]锦州医科大学附属第三医院肿瘤科,辽宁锦州121000

出　　处：《中国医科大学学报》2017年第5期422-424,428,共4页Journal of China Medical University

基　　金：辽宁省自然科学基金(2015020366)

摘　　要：目的检测A549中色素上皮源性因子(PEDF)通过血管内皮生长因子(VEGF)影响细胞的增殖与迁移的方式。方法过表达或沉默PEDF后观察其对VEGF的影响。MTT与trasnwell检测PEDF对细胞增殖和迁移的作用。实时PCR及Western blotting检测PEDF如何调节VEGF/SRC/FAK通路。结果 PEDF可抑制VEGF,进而抑制A549细胞的增殖与迁移。结论PEDF可能成为肺癌的潜在诊疗靶点。Objective To analyze the mechanism of pigment epithelium-derived factor （PEDF） regulation in lung cancer cell proliferation and migration by vascular endothelial growth factor （VEGF）. Methods VEGF expression was observed after overexpression or silencing of PEDF. Proliferation and migration were analyzed by MTT and transwell assays. Real-time PCR and Western blotting were performed to investigate the mechanism underlying PEDF regulation of the VEGF/SRC/FAK pathways. Results PEDF could inhibit the proliferation and migration of A549 cells by VEGF. Conclusion PEDF can be considered as a potential therapeutic target for lung cancer.

关 键 词：非小细胞肺癌 血管内皮生长因子 A549 色素上皮源性因子 

分 类 号：R734.2[医药卫生—肿瘤]