补阳还五汤对失神经肌萎缩大鼠NF-κB/MuRFl表达的影响  

Effect of Buyang Huanwu decoction on NF-κB/MuRFl expression in rats with denervated tibial muscle atrophy

作　　者：周岚[1] 梅晓云[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210023

出　　处：《中国中药杂志》2017年第9期1762-1765,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(81302890);江苏省高校自然科学基金面上项目(13KJB360003);江苏省自然科学基金面上项目(BK2011816);高等学校博士学科点专项科研基金面上项目(新教师类)(20123237120004);南京中医药大学青年自然科学基金项目(12XZR09)

摘　　要：为了研究补阳还五汤(黄芪、当归、白芍、地龙、川芎、红花、桃仁)对失神经肌萎缩大鼠NF-κB/MuRFl信号通路的影响,探讨其对失神经肌萎缩的保护机制,该实验建立大鼠腓总神经夹伤模型。将造模后的60只SPF级SD大鼠随机分为:假手术组、补阳还五汤高、中、低剂量组、弥可保组与模型组,术后每日灌胃给药。10 d与21 d后qRT-PCR与Western blot检测NF-κB,MuRFl基因与蛋白的差异表达。结果显示:与模型组相比,补阳还五汤中、高剂量组NF-κB,MuRFl mRNA与蛋白表达均显著降低(P<0.05或P<0.01)。提示:补阳还五汤可能通过拮抗NF-κB/MuRFl信号通路,抑制蛋白酶体系统导致的蛋白质降解来防治失神经肌萎缩的发生。We aim to study the effect of Buyang Huanwu decoction（Astragali Radix,Angelicae Sinensis Radix,Paeoniae Radix Alba,Pheretima,Chuanxiong Rhizoma,Carthami Flos and Persicae Semen） on NF-κB/MuRFl signaling pathway in the denervated tibial muscle atrophy of rats and explore its protection mechanisms. Sixty Sprague-Dawley rats were subjected to common peroneal nerve crush models of 5 mm injury,and then were randomly divided into six groups for daily intragastric administration of drugs： sham operation group,model group,Buyang Huanwu decoction high,middle and low dose groups,and mecobalamin group（ as a positive control）. After drug administration for 10 days and 21 days,the gene and protein expression levels of NF-κB and MuRFl in the tibial muscle of rats were detected by qRT-PCR and Western blot respectively. The results showed that,as compared with the model group,RNA and protein expression levels of NF-κB and MuRF1 were significantly reduced in Buyang Huanwu decoction middle and high-dose groups（ P〈0. 05 or P〈0. 01）. These results indicated that Buyang Huanwu decoction could inhibit ubiquitin proteasome system probably by activating NF-κB/MuRF1 signal pathway to protect the denervated tibial muscle atrophy of rats.

关 键 词：补阳还五汤 失神经 肌萎缩 胫前肌 大鼠NF-κB MuRF1 

分 类 号：R285.5[医药卫生—中药学]