补骨脂-肉豆蔻药对配伍前后主要成分的UPLC-MS/MS测定及药代动力学研究  

Determination and pharmacokinetics of main components for Psoralea corylifolia-Myristica fragrants drug pair by using UPLC-MS/MS

作　　者：高家荣[1] 徐双枝 韩燕全[1] 魏良兵[1] 姜辉[1] 宋俊梅[2] 薛雪[1] 

机构地区：[1]安徽中医药大学第一附属医院国家中医药管理局中药制剂三级实验室,安徽合肥230031 [2]安徽中医药大学药学院,安徽合肥230038

出　　处：《中国中药杂志》2017年第9期1782-1786,共5页China Journal of Chinese Materia Medica

基　　金：国家中医药重点学科临床中药学建设项目(国中医药人[2012]32号教发);2016年度省卫生计生委中医药科研课题(2016zy80);安徽中医药大学科学研究基金项目(2015fy003)

摘　　要：采用UPLC-MS/MS多反应监测(MRM)定量分析,以氯霉素为内标物在正离子模式下建立血浆中补骨脂素、异补骨脂素、补骨脂酚及氢二异丁香酚药物浓度的测定方法,研究口服补骨脂-肉豆蔻药对配伍前后主要成分的药代动力学过程。将36只SD大鼠随机分为3组(A组、B组、C组)并分别灌胃补骨脂提取液、补骨脂-肉豆蔻药对提取液、肉豆蔻提取液,于不同时间点采集血浆样品。血浆样品中补骨脂素、异补骨脂素、补骨脂酚和去氢二异丁香酚分别在0.098 125~39.25,0.084 375~33.75,0.046 875~18.75,0.11~2.2 mg·L^(-1)线性关系良好,精密度、稳定性的试验结果表明,该类成分的血药浓度测定方法稳定可靠。采用DAS 2.0计算所得药代动力学参数在配伍前后均有不同程度的差异,结果表明补骨脂和肉豆蔻配伍能影响主要成分在体内的药动学过程,使分布更广泛,代谢消除更快。To conduct multiple-reaction monitoring（MRM） quantitative analysis with ultra-high performance liquid chromatography coupled with mass spectrometry method（ UPLC-MS/MS）,determine the concentrations of psoralen,isopsoralen,bakuchiol and dehydrodiisoeugenol in plasma under positive iron mode with chloramghenicol as internal standard,and investigate the pharmacokinetics process of the main components before and after oral administration of drug pair Psoralea corylifolia-Myristica fragrants. Thirty-six SD rats were randomly divided into three group（ A,B,C） and received P. corylifolia extract,P. corylifolia-M. fragrants extract,and M. fragrants extract respectively by intragastric administration. The plasma samples were collected at different time points. In the plasma samples,psoralen,isopsoralen,bakuchiol and dehydrodiisoeugenol showed good linear relationship within concentration rages of0. 098 125 to 39. 25,0. 084 37 to 33. 75,0. 046 875 to 18. 75,and 0. 11 to 2. 2 mg·L^-1 respectively. The precision and stability results showed that the determination method of plasma concentration for such compositions was stable and reliable. The pharmacokinetic parameters obtained by DAS 2. 0 showed varying differences before and after compatibility. According to the experimental results,the compatibility of P. corylifolia and M. fragrants can significantly impact the pharmacokinetic process of main components,expand their distribution and accelerate their metabolism and elimination in vivo.

关 键 词：补骨脂 肉豆蔻 配伍 药代动力学 UPLC-MS/MS 

分 类 号：R285.5[医药卫生—中药学]