β-环糊精辅助四(对羟基苯基)卟啉与铅(Ⅱ)离子显色反应  

Study on the chromogenic reaction of TOPPH_2 and lead (Ⅱ) assisted by β-CD

作　　者：孙梅[1] 尚楠[1] 刘亚男[1] 高立彬[1] 单虎[2] 李建忠[1] 

机构地区：[1]青岛农业大学化学与药学院,青岛266109 [2]青岛农业大学动物科技学院,青岛266109

出　　处：《分析试验室》2017年第5期593-595,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21404066);山东省自然科学基金(ZR2013BL009);青岛市应用基础研究项目(15-9-1-16-jch);青岛农业大学教学研究(XYX2014014);大学生创新项目资助

摘　　要：利用四(对羟基苯基)卟啉(TOPPH_2)为显色剂,在β-环糊精作用下,研究了TOPPH_2与Pb^(2+)的显色反应。结果显示,在pH 10.0,沸水浴加热15 min,Pb^(2+)与TOPPH_2形成稳定的配合物TOPPPb(Ⅱ),在λ_(max)=436 nm检测吸光度,发现该条件下显色剂吸收峰较宽。当显色液冷却至室温,加入一定量β-环糊精,震荡20 min,调节pH为4.6,发现溶液在426 nm处有较窄的吸收峰,显色反应得到增强,当Pb(NO_3)_2质量浓度范围为0~0.15 mg/L,吸收峰强度与溶液中Pb^(2+)的浓度成正比,符合比尔定律。The chromogenic reaction of TOPPH_2 and lead ion was studied using tetra（ phydroxyphenyl） porphyrin（TOPPH_2） as the chromogenic reagent. Results showed that TOPPPb（ II）complexes were formed when p H of reaction solution was 10. 0 in boiling water bath for 15 min and a broad absorption peak at 436 nm was observed. A narrow absorption peak at 426 nm with a higher absorbance was found when the solution was cooled to room temperature by adding a certain amount ofβ-cyclodextrin solution,adjusting pH to 4. 6 and rocking for 20 min. The color reaction with different concentration of lead nitrate ranging of 0 ～ 0. 15 mg/L was tested,and the results showed that the absorbance varies directly with the concentration of lead ion in accordance with Bill＇s law.

关 键 词：四(对羟基苯基)卟啉 Β-环糊精 分光光度法 铅(Ⅱ) 

分 类 号：O657.3[理学—分析化学]