复发性流产蜕膜组织MIR-21、MIR-31的表达研究  

Expression of MIR-21 and MIR-31 in Decidua of Recurrent Abortion

作　　者：田玉华[1] 张玉杰[1] 叶飞雪 游泽山[2] 

机构地区：[1]惠州市第三人民医院妇产科,广东惠州516000 [2]中山大学附属第一医院东院妇产科,广东广州510700

出　　处：《现代诊断与治疗》2017年第2期197-199,共3页Modern Diagnosis and Treatment

摘　　要：目的探究复发性自然流产蜕膜组织中MIR-21、MIR-31的表达情况。方法研究对象为选取进行清宫的49例女性患者,其中24例复发性自然流产者设为观察组,25例计划外怀孕要求终止妊娠者设为对照组,取两组绒毛及蜕膜组织进行研究,采用实时荧光定量PCR检验蜕膜组织中MIR-21、MIR-31的表达情况,并采用蛋白免疫印迹法检验插头样转录因子P3(FOXP3)的表达情况,对比两组的差异性,并分析MIR-21、MIR-31和FOXP3表达的相关性。结果观察组MIR-21、MIR-31、FOXP3的表达与对照组相比均有显著差异(P<0.05),MIR-21和FOXP3表达增高,而MIR-31表达下降;MIR-21、MIR-31的表达均与FOXP3的表达相关(r=0.593,-0.669,P<0.05)。结论蜕膜组织中MIR-21、MIR-31的表达异常会导致FOXP3基因出现表达异常,可能是导致机体免疫机制紊乱并引发自然流产的重要原因。Objective To explore the recurrent spontaneous abortion decidua tissues circumstances MIR-21, MIR-31＇s. Methods The subjects choose between 2013.1-2014.12 in our hospital Qing of 49 female patients, including 24 cases of recurrent spontaneous abortion observation group, 25 cases outside unplanned pregnancies terminate the pregnancy as the control group, taking expression of the two groups of villi and decidua were studied by real-time quantitative PCR test decidua tissues circumstances MIR-21, MIR-31, and using Western blot test plug-like transcription factor P3 （FOXP3） of situation, compared to the differences between the two groups, and analyze MIR-21, MIR-31 and FOXP3 expression correlation. Results The MIR-21, MIR-31, FOXP3 expression compared with the control group were significantly different （P〈O.05）, MIR 21-and FOXP3 expression increased, whereas MIR-31 decreased expression; MIR-21, MIR -31 expression was correlated with the expression of FOXP3 （r=0.593, -0.669, P〈0.05）. Conclusion decidua during MIR-21, MIR-31 will lead to abnormal expression of FOXP3 gene appears abnormal expression may be an important cause of the body＇s immune system disorder and lead to spontaneous abortion.

关 键 词：复发性自然流产 蜕膜组织 MIR-21 MIR-31 

分 类 号：R730.264[医药卫生—肿瘤]