白藜芦醇预处理提高衰老牙周膜干细胞克隆和增殖潜能的研究  

Resveratrol pretreatment promotes in vitro proliferation of periodontal ligament stem cells derived from aged donors

作　　者：胡超 祝金浩 殷园 贺小涛 李璇 张曦予 陈发明(指导) 

机构地区：[1]第四军医大学口腔医院牙周科,军事口腔医学国家重点实验室,口腔疾病国家临床医学中心,陕西省口腔生物工程技术研究中心,陕西西安710032 [2]解放军77626部队医院,西藏拉萨851400 [3]不详

出　　处：《牙体牙髓牙周病学杂志》2017年第5期254-260,共7页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金(81500853)

摘　　要：目的:观察白藜芦醇预处理能否衰使老牙周膜干细胞(Aged-PDLSCs)的增殖潜能和克隆形成能力得到增强。方法:将体外分离培养的中老年个体来源PDLSCs随机分为常规培养组、白藜芦醇预处理组、尼克酰胺预处理组;各组经相应处理后,采用MTT法检测其增殖潜能,结晶紫染色检测其克隆形成能力,RT-PCR和Western Blot检测其细胞中SIRT1和P53基因及蛋白的表达水平。结果:Aged-PDLSCs经白藜芦醇预处理后增殖潜能和克隆形成能力均得到了显著提高,而经尼克酰胺预处理后,其增殖潜能和克隆形成能力均明显降低;分别与常规培养组相比,差异均有统计学意义(P<0.05)。此外,白藜芦醇预处理还可显著提高Aged-PDLSCs中SIRT1活性(P<0.05),显著降低了P53的表达水平(P<0.05)。结论:白藜芦醇能够增强Aged-PDLSCs的克隆形成能力和增殖潜能,其作用可能是通过提高SIRT1的表达水平而实现的。AIM To investigate whether resveratrol （ AV） can promote the proliferation and colony - form-ing of periodontal ligament stem cells （PDLSCs） derived from aged donors. METHODS： The PDLSCs were acquired from the periodontal ligament tissues of middle - aged and elderly subjects by enzymolysis and cultured in vitro, the PDLSCs were not pretreated（control group） or pretreated by AV （AV group） or nicotinamide（NT group）. Cell prolif-eration was determined by MTT assay and colony formation was detected by crystal violet staining. PCR and Western blotting were performed to determine the relative expressions of SIRT1 and HIFl-a. RESULTS ： AV pretreatment en-hanced the capacities of proliferation and colony formation of PDLSCs derived from aged donors （P 〈0. 05） , while NT impaired the proliferation and colony formation of the PDLSCs （P 〈 0. 05 ） . The expression of SIRT1 was promoted and the expression of P53 was down - regulated by AV pretreatment （P 〈 0. 05 ） . CONCLUSION ： Resveratrol can reverse the proliferation and colony formation of PDLSCs derived from aged donors via the activation of SIRT1.

关 键 词：牙周膜干细胞 白藜芦醇 尼克酰胺 衰老 SIRT1 

分 类 号：R780.2[医药卫生—口腔医学]