多巴酚丁胺联合顺铂对胃癌移植瘤YAP和p-YAP蛋白表达的影响  

Inhibitory Effect of Dobutamine Combined with Cisplatin on Gastric Implant Tumor and Expression of YAP and p-YAP

作　　者：樊静华[1] 高小云[1] 郑绘霞[2] 肖虹[2] 武丽娜[2] 梁钢[2] 梁建芳[2] 

机构地区：[1]山西医科大学基础医学院,山西太原030001 [2]山西医科大学第一临床医院

出　　处：《中国医学创新》2017年第14期28-31,共4页Medical Innovation of China

摘　　要：目的:通过建立胃癌移植瘤模型,观察多巴酚丁胺联合顺铂对胃癌移植瘤的生长抑制作用及对瘤体组织中YAP、p-YAP表达的影响。方法:采用人胃癌SGC-7901细胞株建立裸鼠皮下移植瘤模型,随机分为5组:空白对照(CK)组、阴性对照(NS)组、多巴酚丁胺(DOB)组、顺铂(DDP)组、联合用药(DOB+DDP)组。治疗结束后摘除移植瘤组织,测量并称重,计算抑瘤率。通过Western Blot检测YAP和p-YAP蛋白的表达。结果:治疗结束后CK、NS、DOB、DDP、DOB+DDP组瘤体终体积依次为(472.09±9.44)、(451.94±16.32)、(410.14±15.39)、(246.69±15.54)和(263.42±19.55)mm^3。各组瘤质量分别为(0.84±0.02)、(0.82±0.04)、(0.65±0.03)、(0.35±0.01)和(0.35±0.02)g,其中实验组(DOB、DDP、DOB+DDP)抑瘤率分别为22.67%、58.71%和58.47%,与对照组(CK、NS)相比,差异均有统计学意义(P<0.05)。DOB组和DDP组YAP表达量均低于对照组(P<0.05),p-YAP表达量高于对照组(CK、NS)(P<0.05),DOB+DDP组与对照组(CK、NS)相比差异均无统计学意义(P>0.05)。结论:多巴酚丁胺可能通过下调YAP,同时上调p-YAP的表达抑制胃癌移植瘤生长,与顺铂联合抑制作用不明显。Objective： To investigate the effects of Dobutamine combined with Cisplatin on the growth of human gastric tumor SGC-7901 cell xenografts in nude mice and the possible mechanisms involved in the expression of YAP and p-YAP.Method： The human gastric cancer SGC-7901 cell line subcutaneous transplantation tumor mode were randomly divided into 5 groups ： Blank control （ CK ） group, Negative control （ NS ） group, Dobutamine （ DOB ） group, Cisplatin （ DDP ） group, Dobutamine±Cisplatin （ DOB±DDP ） group.After the treatment to remove the transplanted tumor tissue, tumor volume and weight were observed .The expression of YAP and p-YAP were evaluated by Western Blotting analysis.Result： Tumor volumes in CK group, NS group, DDP group, DOB group and DDP±DOB group were （472.09 ± 9.44 ）, （ 451.94 ± 16.32 ）, （ 410.14 ± 15.39 ）, （ 246.69 ± 15.54 ） and （ 263.42 ± 19.55 ） mm3.The quality of groups, respectively were （ 0.84 ± 0.02 ）, （ 0.82 ± 0.04 ）, （ 0.65 ± 0.03 ）, （ 0.35 ± 0.01 ） and （ 0.35 ± 0.02 ） g.Inhibitory rate of the experimental groups were 22.67%, 58.71% and 58.47%, the differences were statistically significant compared with the control groups （ P〈0.05 ） .Compared with the control groups, YAP expressing quantity of the experimental groups was lower （ P〈0.05 ）, p-YAP expressing quantity was higher （ P〈0.05 ） .Conclusion： Dobutamine may through cutting YAP and raising p-YAP expression inhibit the growth of gastric cancer xenografts in nude mice, while Cisplatin joint inhibitory effect is not obvious.

关 键 词：胃癌 多巴酚丁胺 顺铂 YAP p-YAP 

分 类 号：R735.2[医药卫生—肿瘤]