TUFT1真核表达质粒的构建及其功能研究  

Research on Construction of TUFT1 Eukaryotic Expression Plasmid and Its Function

作　　者：袁孔现[1] 王继年[2] 

机构地区：[1]铜陵市人民医院药剂科,安徽铜陵244002 [2]安徽医科大学第一附属医院教育处,安徽合肥230032

出　　处：《皖西学院学报》2017年第2期71-74,共4页Journal of West Anhui University

摘　　要：设计合成引物,扩增出TUFT1的CDS序列,双酶切后连接到pEGFP-C2载体上,将过表达质粒转染至胰腺癌细胞株中。结果显示,过表达组细胞的增殖率,显著高于空白组和对照组;过表达组细胞的凋亡率,显著低于空白组和对照组。成功构建TUFT1的真核表达载体,并初步确定TUFT1可以明显增强人胰腺癌细胞株的增殖,同时抑制其凋亡,为进一步探索TUFT1基因功能、寻求治疗胰腺癌的新途径提供了细胞学基础。Extract total RNA of human hepatic stellate cells, reverse transcription into cDNA as templates, and obtain amplification TUFT1 CDS sequence, after connect to pEGFP-C2 carrier by using the double enzyme. Eukaryotic expression plasmid was transfected respectively to human pancreatic cancer cell line. Then, result showed that the cell proliferation rate of transfection group was significantly lower than normal group; cell apoptosis rate of transfection group was significantly higher than normal group. TUFT1 can significantly inhibit the proliferation of pancreatic cancer cell line, and promote its apoptosis. These results have a further understanding the function of TUFT1, and lay a foundation for searching a new direction in the therapy of pancreatic cancer.

关 键 词：TUFT1 转染 增殖 凋亡 

分 类 号：R735.9[医药卫生—肿瘤]