高效液相色谱法测定山楂叶中的9种酚类成分  

Determination of nine phenolic components in the leaves of Chinese hawthorn by HPLC

作　　者：杨晓博[1] 王荣芳[1] 贾亚楠[1] 毕振良 陆凤琴 崔同[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071000 [2]兴隆县林业局,河北承德067000

出　　处：《食品工业科技》2017年第10期62-66,共5页Science and Technology of Food Industry

基　　金：河北省自然科学基金(C2015204187)

摘　　要：利用反相高效液相色谱法同时测定山楂叶中的9种酚类成分。结果表明,9种酚类成分在2~500μg/m L之间呈良好的线性关系,r介于0.9995~0.9999。加标回收率为93.7%~110.2%,相对标准偏差在0.69%~4.58%之间。对29个品种的山楂叶进行测定,9种酚类成分的平均含量由低到高依次为:异槲皮苷、金丝桃苷、原花青素C1、原花青素D1、表儿茶素、原花青素B2、绿原酸、红果酸、牡荆素鼠李糖苷。其中黄酮和酚酸含量较高,达15 mg/g D.W左右,原花青素类成分含量达6 mg/g D.W。该方法具有简便、准确的优点。Nine phenolic components in the leaves of Chinese hawthorn were determined by RP-HPLC simultaneously. The results indicated that the linearity ranges of nine compounds were between 2-500 p.g/mL and the correlation coefficient （r） were between 0.9995-0.9999.The recovery was 93.7%-110.2% .The relative standard deviations were between 0.69% -4.58%. The average content of nine phenolic components from low to high were isoquercitrin,hyperoside,procyanidin C1 , procyanidins D 1, epicatechin, proanthocyanidin B2, chlorogenic acid, eucomic acids, vitexin- 2＂- O- rhamnoside. Among them, the content of flavonoids and phenolic acids was higher than 15 mg/g D.W and the content of proanthocyanidins was up to 6 mg/g D.W.The method of HPLC was simple and accurate.

关 键 词：高效液相色谱法 山楂叶 原花青素 红果酸 黄酮 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]