软骨分化间充质干细胞对颞下颌关节炎软骨修复的影响  

Effect of chondrogenic differentiation of autologous mesenchymal stem cells on cartilage repair in osteoarthritis of temporomandibular joint in vitro

作　　者：王鹏[1] 陈瞰[1] 任笑春 叶斌[1] 李运峰[1] 祝颂松[1] 

机构地区：[1]口腔疾病研究国家重点实验室,国家口腔疾病临床研究中心,四川大学华西口腔医院,四川成都610041

出　　处：《临床口腔医学杂志》2017年第4期203-207,共5页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助(81271170)

摘　　要：目的:探讨软骨分化的间充质干细胞对颞下颌关节炎软骨修复的影响。方法:将诱导出骨关节炎模型4周后的新西兰白兔46只,关节内分别注射hylartin溶液(安慰剂组,A组)、非软骨分化的间充质干细胞(B组)、软骨分化的间充质干细胞(C组)。注射后的4、12、24周观察软骨的组织学变化。结果:与第4周相比,术后第12周软骨分化间充质干细胞组的软骨分化有较好的组织学表现,但这一差别在第24周时变小。术后第4周,可在软骨及关节囊中检测到注射的细胞。结论:关节腔内注射间充质干细胞可延缓颞下颌关节炎软骨病变的进展,且体外软骨向诱导的间充质干细胞可在更大程度上提高其治疗效果。Objective： This study investigated the effects of chondrogenic differentiated mesenchymal stem cells （MSCs） on cartilage in temporomandibular joint osteoarthritis（TMJOA） in vitro. Methods ： Four weeks after induction of osteoarthritis（OA） ,the joints received hylartin solution, non-chondrogenic MSCs or in vitro chondrogenie differentiated MSCs. The changes in cartilage were evaluated by histology, reverse transcription polymerase chain reaction. Results：The differentia- ted MSC-treated group had better histology than the MSC-treated group at 4 and 12 weeks, but with no difference at 24 weeks. Transplanted cells were observed at cartilage and the synovial membrane lining at 4 weeks. Conclusion：Intraarticular injection of MSCs could delay the progression of TMJOA,and in vitro chondrogenic induction of MSCs could enhance the therapeutic effects.

关 键 词：骨髓间充质干细胞 软骨细胞分化 颞下颌关节 骨性关节炎 软骨 

分 类 号：Q813.1[生物学—生物工程] R782.6[医药卫生—口腔医学]