不同周龄SD大鼠肝脏非肿瘤病变分析  

Analysis of non-neoplastic hepatic lesions in SD rats at different age

作　　者：高洪彬[1] 宋向荣[1] 黄振烈[1] 蔡婷峰[1] 陆丰荣[1] 殷霄[1] 陈晓燕[1] 马超亚[1] 黄曼琪 郑杰蔚 谢植伟[1] 曾丽海[1] 梁旻炜 陈润涛[1] 

机构地区：[1]广东省职业病防治院,广东省职业病防治院重点实验室,广东广州510300

出　　处：《中国职业医学》2017年第2期159-163,共5页China Occupational Medicine

基　　金：广东省医学科学技术研究基金(A2016122);广东省职业病防治重点实验室(2012A061400007)

摘　　要：目的探讨不同周龄SD大鼠肝脏非肿瘤病变情况。方法收集亚急性毒性实验、亚慢性毒性实验和慢性毒性与致癌合并实验安全性评价毒理学实验中空白对照组的无特定病原体级SD大鼠,于实验终期即大鼠周龄分别为第10、19、56和108周龄(分别设为10、19、56和108周龄组,每组雌、雄各100只)时处死大鼠,计算肝脏脏器系数,并进行肝脏组织病理诊断,分析不同周龄大鼠肝脏非肿瘤病变情况。结果大鼠肝脏脏器系数在19、56和108周龄组均低于10周龄组(P<0.05),在56和108周龄组均低于19周龄组(P<0.05),在108周龄组高于56周龄组(P<0.05)。10、19、56和108周龄组大鼠肝脏非肿瘤病变在雌性大鼠分别检出6、6、13和15种,在雄性大鼠分别检出6、8、12和17种。无论是雌性还是雄性大鼠,各组大鼠肝细胞脂肪变性、肝细胞水肿和肝脏炎症细胞浸润的发生率均随着周龄的增加而增加(P<0.05);56、108周龄组大鼠肝内胆管增生和肝内胆管纤维化的发生率均高于10、19周龄组(P<0.008);108周龄组大鼠肝窦扩张的发生率高于19周龄组(P<0.008)。结论 SD大鼠肝脏自发性非肿瘤病变较多见,以肝细胞脂肪变性、肝细胞水肿和肝脏炎症细胞浸润为主,且其发生率呈随大鼠周龄的增加而增加的趋势。Objective To explore the non-neoplastic hepatic lesions in SD rats at different ages.Methods The specific-pathogen free SD rats were collected from the blank control groups used in subacute toxicity tests,subchronic toxicity tests and chronic toxicity combined with carcinogenic tests for safety evaluation.At the end of each experiment,i.e.week 10,19,56 and 108 （assigned into four groups： 10,19,56 and 108 weeks,each contained 100 rats with each sex）,rats were executed.The liver organ coefficient was calculated,the pathological examination was performed,and the non-tumorous lesions in the liver were analyzed.Results The liver organ coefficients at the age of 19,56,108 weeks were lower than that of 10 weeks （P〈0.05）;those at the age of 56 and 108 weeks were lower than that of 19 weeks （P〈0.05）,and that of 108 weeks was greater than of 56 weeks （P〈0.05）.Among the 10-week-old,19-week-old,56-week-old and 108-week-old groups,the types of non-neoplastic hepatic lesions detected in the female rats were 6,6,13 and 15 respectively,meanwhile those in the male rats were 6,6,13 and 15 respectively.Both male and female rats,the incidences of hepatocyte fatty degeneration,edema and hepatic infiltration of inflammatory cells were significantly increased with the increase of age in each group （P〈0.05）.The incidences of intrahepatic bile duct proliferation and intrahepatic bile duct fibrosis in rats at the age of 56 and 108 weeks were higher than those at the age of 10 and 19 weeks （P〈0.008）.Moreover,the frequency of hepatic sinus expansion lesions in rats at the age of 108 weeks was higher than those of 19 weeks （P〈0.008）.Conclusion Spontaneous non-neoplastic lesions in the liver of SD rats were common,primarily demonstrated as hepatocyte fatty degeneration,edema and infiltration of inflammatory cells.The incidences of lesions increased with the increase of age.

关 键 词：SD大鼠 肝脏 非肿瘤病变 脏器系数 发生率 

分 类 号：R737.9[医药卫生—肿瘤] R114[医药卫生—临床医学]