HPLC-UV同时测定高粱红色素中两种成分的含量  

Simultaneous determination of two sorghum pigment components by HPLC-UV

作　　者：胡晓琴[1] 韩春[1] 

机构地区：[1]长治学院化学系,长治046011

出　　处：《中国食品添加剂》2017年第4期157-162,共6页China Food Additives

基　　金：国家自然科学基金青年基金项目(No.21402012);国家自然科学项目子项目(No.GJZXM201605)

摘　　要：建立高效液相色谱法测定高粱红色素中芹菜素和槲皮素-7-葡萄糖苷的分析方法。样品经前处理提取溶解,采用C_(18)反相色谱柱分离,用甲醇-1%乙酸溶液(60∶40,v/v)为流动相进行洗脱,检测波长为377nm,流速为1.0 m L/min,以色谱峰保留时间进行定性,外标法进行定量。结果表明,芹菜素和槲皮素-7-葡萄糖苷分别在10.2~204.0μg/m L和20.4~408.0μg/m L范围内线性关系良好,相关系数R2均大于0.999,样品中的加标回收率分别为94.3%~101.3%和89.4%~101.6%,相对标准偏差分别为1.8%~7.9%和2.1%~4.2%(n=3),检出限分别为2μg/mg和4μg/mg,该方法操作简单,精密度好,能满足高粱红色素中芹菜素和槲皮素-7-葡萄糖苷的检测要求。A new method for the determination of apigenin and quercetin-7-glucoside in sorghum red was developed by HPLC.The sample was extracted with ethylalcohol-HCl（pH3.0）.The chromautographic separation was performed on an RP-C_（18） column,the mobile phase consists of methanol and 1 % acetic acid（60 ∶ 40,v/v）and flow at 1.0 m L/min.The detection wavelength was 377 nm,the retention time was used in qualitative analysis.The external standard method was used in quantitative analysis.The method showed good linearity in the range of 10.2 ~ 204.0 μg/m L for apigenin,and 20.4 ~ 408.0 μg/m L for quercetin-7-glucoside with correlation coefficients（R2）all above 0.999.The recoveries were 94.3 % ~ 101.3 % and 89.4 % ~ 101.6 % with relative standard deviations（RSDs,n=3）of 1.8% ~ 7.9% and 2.1% ~ 4.2%,respectively.The limits of detection（LODs）were 2 μg/mg and 4 μg/mg,respectively.The method was simple with good precision and suitable for the determination of apigenin and quercetin-7-glucoside in sorghum red.

关 键 词：高粱红 槲皮素-7-葡萄糖苷 芹菜素 色素 高效液相色谱法 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程]