Targeting BCRP/ABCG2 by RNA Interference Enhances the Chemotherapy Sensitivity of Human Colon Cancer Side Population Cells  

作　　者：胡均 李健 岳欣 王家仓 王俊锋 柳建中 孔大陆 

机构地区：[1]Department of Colorectal Cancer Surgery,National Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy of Tianjin,Tianjin's Clinical Research Center for Cancer,Tianjin Medical University Cancer Institute and Hospital [2]Department of Lymphoma,State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2017年第2期231-236,共6页华中科技大学学报（医学英德文版）

基　　金：supported by grants from the National Nature Science Foundation of China(No.81101870);the National Key Clinical Specialist Construction Programs of China(No.2013-544);the Key Programs of National Health and Family Planning Commission of Tianjin(No.16KG127)

摘　　要：Relapse and metastasis are frequent in colon cancer and may be linked to stem cell characteristics.This study isolated side population（SP） cells from a colon cancer cell line（Colo-320） and examined their self-renewal and differentiation abilities.Compared to non-SP（NSP） cells,SP colon cancer cells were more tumorigenic in vivo and exhibited more invasive characteristics and a greater ability to form colonies.Additionally,more cells were in G0/G1 phase and more highly expressed the multidrug resistance protein BCRP/ABCG2.We achieved enhanced chemotherapy sensitivity by transfecting SP cells with a hairpin-like,small interfering RNA（si RNA） eukaryotic expression plasmid targeting BCRP/ABCG2.Relapse and metastasis are frequent in colon cancer and may be linked to stem cell characteristics.This study isolated side population（SP） cells from a colon cancer cell line（Colo-320） and examined their self-renewal and differentiation abilities.Compared to non-SP（NSP） cells,SP colon cancer cells were more tumorigenic in vivo and exhibited more invasive characteristics and a greater ability to form colonies.Additionally,more cells were in G0/G1 phase and more highly expressed the multidrug resistance protein BCRP/ABCG2.We achieved enhanced chemotherapy sensitivity by transfecting SP cells with a hairpin-like,small interfering RNA（si RNA） eukaryotic expression plasmid targeting BCRP/ABCG2.

关 键 词：side population cells colon cancer chemotherapy sensitivity RNAi bcrp/abcg2 

分 类 号：R735.35[医药卫生—肿瘤]