Oct4与TGF-β信号通路相关因子在胃癌组织中的表达和相互关系  

EXPRESSION AND CORRELATIONS OF OCT4 AND TGF-Β SIGNALING PATHWAY RELATED FACTORS IN GASTRIC CANCER

作　　者：蔡梦月[1] 郝美玲[1] 李春辉[1] 

机构地区：[1]承德医学院附属医院,河北承德067000

出　　处：《承德医学院学报》2017年第3期185-187,共3页Journal of Chengde Medical University

基　　金：2014年度河北省医学科学研究重点课题及河北省政府资助临床优秀人才基金资助(ZL20140103)

摘　　要：目的:探讨八聚体结合蛋白4(Oct4)与TGF-β信号通路相关因子在胃癌组织中的表达情况和相互关系。方法:57例胃癌患者手术切除的癌组织及对应正常胃组织,采用Western blotting法检测Oct4、TβRⅡ、Smad2、Smad3、E-Cadherin蛋白的表达情况,分析它们与胃癌临床病理特征的关系,以及胃癌组织Oct4表达与TβRⅡ、Smad2、Smad3、E-Cadherin的相关性。结果:胃癌组织Oct4蛋白的表达明显高于正常组织,TβRⅡ、Smad2、Smad3、E-Cadherin蛋白的表达明显低于正常组织(P<0.05)。胃癌组织Oct4、TβRⅡ、Smad2、Smad3、E-Cadherin表达与与肿瘤组织分型、临床分期、淋巴结转移和浆膜浸润有关(P<0.05)。胃癌组织Oct4表达与TβRⅡ、Smad2、Smad3、E-Cadherin呈负相关关系(r=-0.887、-0.912、-0.779、-0.879,P<0.01)。结论:胃癌组织Oct4高表达可参与胃癌的发生发展和浸润、转移,其机制可能与调节TGF-β信号通路,诱导细胞上皮间质转化有关。Objective:To investigate the expression and correlations of Oct4 and TGF-β signaling pathway related factors in gastric cancer.Methods:Western blotting was used to detect the expression of Oct4,E-Cadherin and TGF-β signaling pathway related factors TβRⅡ,Smad2,Smad3 protein in 57 cases of gastric cancer and normal gastric tissue;as well as the relationships between above-mentioned factors and clinicopathologic feature of gastric cancer,and the correlations between Oct4 expression and TGF-β signaling pathway related factors were also analyzed.Results:Compared with normal gastric tissue,the Oct4 expression in gastric cancer increased obviously,while the TβRⅡ,Smad2,Smad3,E-Cadherin expression decreased obviously(P<0.05).In gastric cancer,the expression of Oct4,TβRⅡ,Smad2,Smad3,E-Cadherin were related to tumour tissue typing,clinical stage,lymph node metastasis and serosal invasion(P<0.05).In gastric cancer,the Oct4 expression was negatively related to TβRⅡ,Smad2,Smad3 and E-Cadherin(r=-0.887,-0.912,-0.779,-0.879,P<0.01).Conclusions:High expression of Oct4 in gastric cancer may be involved in the development,invasion and metastasis of gastric cancer.The mechanisms may be related to induced epithelial mesenchymal transition by regulating TGF-β signaling pathway.

关 键 词：胃癌 八聚体结合蛋白4(Oct4) TGF-Β信号通路 上皮间质转化 

分 类 号：R735.2[医药卫生—肿瘤]