基于16S核糖体DNA研究中国南方人群结直肠腺瘤患者肠道菌群特征  

Characteristics of gut microbiota in southern Chinese population with colorectal adenoma based on 16S ribosomal DNA

作　　者：王文佳[1] 何杰[1] 周有连[1] 冯志强[1] 张龙[1] 赖晓波[1] 王红[1] Wang Wenjia He Jie Zhou Youlian Feng Zhiqiang Zhang Long Lai Xiaobo Wang Hong(Department of Gastroenterology, Digestive Disease Center, First Municipal People＇ s Hospital A]filiated to Guangzhou Medical University, Guangzhou, Guangdong 510180, China)

机构地区：[1]广州医科大学附属市一人民医院消化疾病中心消化内科,广州510180

出　　处：《中华生物医学工程杂志》2016年第6期470-475,共6页Chinese Journal of Biomedical Engineering

基　　金：广东省科技计划项目（2014A020212568）

摘　　要：目的 探讨中国南方人群结直肠腺瘤患者肠道菌群结构和多样性特征.方法 提取标本宏基因组DNA,扩增V4可变区后采用细菌16S核糖体DNA（rDNA）高通量测序技术及生物信息学方法,分析36例健康志愿者及49例结直肠腺瘤患者的腺瘤、腺瘤旁黏膜相关性肠道菌群的多样性;门、科、属、种水平的分布特征和生物学标记.结果 健康对照组与结直肠腺瘤旁组的黏膜相关性肠道菌群多样性和菌群分布相似（P〉0.05）;结直肠腺瘤组黏膜相关性肠道菌群多样性明显下降（P〈0.05）,菌群分布主要表现为厚壁菌门、拟杆菌门、毛螺旋菌属、Blautia属、粪球菌属、卵形拟杆菌种丰度显著下降（P〈0.05）,而变形菌门、盐单胞菌科和海藻希万氏菌属丰度明显增加（P〈0.05）.结论 高通量测序证实结直肠腺瘤患者有独特的菌群多样性和特征,提示特征性变化的肠道菌群可能参与结直肠腺瘤的发生过程.Objective To investigate the structure and diversity of gut microbiota in southern Chinese population with colorectal adenoma. Methods The metagenomic DNA of the samples was extracted,and the V4 variable region was amplified. Using the 16S ribosomal DNA （rDNA） high-throughput sequencing and bioinformatics,we analyzed the diversity of adenoma and para-adenoma mucosa-associated gut microbiota in 36 healthy volunteers and 49 patients with colorectal adenoma. The distribution and biological markers of the phyla,families,genuses and species of gut microbiota were also analyzed. Results The diversity and distribution of mucosa-associated gut microbiota in healthy control group and colorectal adenoma group were similar（P〉0.05）. The diversity of mucosa-associated gut microbiota in the colorectal adenoma group significantly decreased （P〈0.05）. The distribution analysis of the gut microbiota showed significant reduction in the abundance of Firmicutes, Bacteroidetes, Lachnospira, Blautia, Coprococcus,Bacteroides-ovatus （P〈0.05）,and increase in the abundance of Proteobacteria phylum, Halomonadaceae and Shewanella-algae（P〈0.05）. Conclusion High-throughput sequencing confirms that there are specific diversity and characteristics of gut microbiota in patients with colorectal adenoma , suggesting that the change in characteristics of the gut microbiota may be involved in the occurrence of colorectal adenoma.

关 键 词：DNA 核糖体 腺瘤 黏膜 细菌 

分 类 号：R735.34[医药卫生—肿瘤]