Sprouty-2对胃癌细胞上皮间质转化及侵袭转移的影响  

Effect of Sprouty-2 on the Epithelial-mesenchymal Transition, Migration and Invasion of Human Gastric Cancer

作　　者：温伟伟[1] 李洪波[1] 姜英健[1] 秦凯强[1] 梁忆波[1] 马雷[1] 郑红梅[1] 张佃良[1] 

机构地区：[1]青岛大学青岛市市立医院结直肠中心,山东青岛266011

出　　处：《现代生物医学进展》2017年第17期3250-3254,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81270448;81470890)

摘　　要：目的:研究Sprouty2(SPRY2)基因在胃癌肿瘤细胞上皮间质转化(EMT)和侵袭转移的影响。方法:体外培养人胃癌细胞(BGC-823),采用慢病毒介导的sh RNA沉默SPRY2基因,并用实时定量PCR与Western blot检测其SPRY2、E-钙黏蛋白(E-cadherin)、波形蛋白(vimentin)的表达,采用细胞划痕实验、Transwell实验检测SPRY2基因沉默后的胃癌细胞侵袭转移能力变化。结果:在慢病毒介导sh RNA沉默SPRY2基因的人胃癌BGC-823细胞中,SPRY2的m RNA和蛋白表达明显降低(P<0.05),SPRY2沉默后人胃癌细胞E-cadherin的蛋白表达增多(P<0.05),vimentin的蛋白表达减少(P<0.05)。此外,SPRY2沉默后,胃癌细胞迁移能力和侵袭能力明显减弱(P值均P<0.05)。结论:Sprouty-2基因通过调节E-cadherin与vimentin的表达参与胃癌细胞的上皮-间质转化,进而促进胃癌细胞的迁移与侵袭。Objective： To investigate the effect of suppressed Sprouty2 （SPRY2） on the epithelial-mesenchymal transition （EMT）, migration and invasion of human gastric cancer. Methods： Human gastric cancer line （BGC-823） was transfected with shRNA-SPRY2 mediated by recombinant lentivirus to establish a cell line （shRNA- SPRY2- BGC-823） which was stably suppressed to express SPRY2. The expression level of SPRY2, EMT-related marker proteins （E-cadherin and vimentin） were examined using real-time PCR and West- ern blot in human BGC-823 cells. In addition, migration and invasion assays were performed to assess the effect of SPRY2 on the BGC-823 cells using Matrigel-coated plate, transwell membrane chamber, and wound healing models, respectively. Results： The human gastric cancer line （shRNA- SPRY2- BGC-823） was successfully constructed by lentivirus stable transfection to suppress the expression levels of SPRY2. The expression of SPRY2 was significantly decreased after knockdown of SPRY2 in BGC-823 cells compared with control-shRNA and non-transfection. The results from real-time PCR and Western blot indicated that the mRNA and protein expression of the epithelial cell marker E-eadherin was significantly increased, and the mesenchymal cell markers vimentin was significantly decreased after knockdown of SPRY2 in BGC-823 cells. Moreover, after SPRY2 knockdown, abilities of migration and invasion were significantly attenuated in shRNA- SPRY2- BGC-823 cells. Conclusions： SPRY2 knockdown significantly promote the EMT by regulating the expressions of E-cadherin and vimentin, which further enhanced the migration and invasion of human gastric cancer in vitro.

关 键 词：胃癌 Sprouty-2 上皮-间质转化 侵袭 转移 

分 类 号：R-33[医药卫生] R735.2