Nat Chem Biol:利用CRISPR-Cas9激活细菌中沉默的基因簇,有望发现新的药物  

CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

作　　者：Mingzi M Zhang, Wan Lin Yeo, Ee Lui Ang, Huimin Zhao Fong Tian Wong, Elena Heng Yajie Wang, Shangwen Luo, Ryan E Cobb, Behnam Enghiad, Huimin Zhao Yee Hwee Lim 

机构地区：[1]Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology, and Research (A*STAR), Singapore [2]Molecular Engineering Lab, Biomedical Science Institutes, A*STAR, Singapore [3]Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA [4]Organic Chemistry, Institute of Chemical and Engineering Sciences, A*STAR, Singapore.

出　　处：《现代生物医学进展》2017年第17期I0004-I0004,共1页Progress in Modern Biomedicine

摘　　要：为了抵抗疾病，很多医药库中的武器是从细菌当中获得的。如今，利用CRISPR-Cas9基因编辑技术。研究人员揭示出沉默基因中隐藏着的更多潜在的宝藏。作为一类常见的细菌。链霉菌被用来产生很多作为抗生素、抗癌试剂和其他药物的化合物。在一项新的研究中。来自美国伊利诺伊大学和新加坡科技研究局的研究人员利用CRISPR-Cas9技术激活链霉菌中不表达的或者说沉默的基因簇。Here we report an efficient CRISPR-Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.

关 键 词：基因簇 CHEM 细菌 利用 激活 药物 NAT 编辑技术 

分 类 号：Q78[生物学—分子生物学]