抑制miR-691通过调控VIM对人胰腺癌BXPC-3细胞侵袭转移的影响  

Inhibition of miR-691 through the regulation of VIM on effects of invasion and metastasis of human pancreatic cancer cell line BXPC-3

作　　者：何燕浙[1] 唐德军[1] 

机构地区：[1]合肥市第三人民医院普外科,合肥230022

出　　处：《肝胆外科杂志》2017年第2期151-154,共4页Journal of Hepatobiliary Surgery

摘　　要：目的探讨微小RNA-691(miR-691)在胰腺癌细胞系中表达状况及抑制miR-691对人胰腺癌BXPC-3细胞生物学行为的影响。方法 miR-691采用实时荧光定量PCR检测。人工化学合成抑制miR-691并转染BXPC-3细胞。流式细胞术检测细胞凋亡情况。通过Transwel^x室细胞侵袭转移实验检测BXPC-3细胞侵袭转移能力。结果人胰腺癌细胞系中BXPC-3对miR-691呈高表达;转染抑制miR-691后,BXPC-3细胞miR-691表达下降,其细胞凋亡无显著改变(P>0.05);而转染抑制miR-691组中通过Transwel小室的细胞数明显高于空白组和对照组(P<0.01)。结论 miR-691可有望成为人胰腺癌基因治疗的候选靶点。Objective To study the small RNA-691 （miR-691） expression in human pancreatic cancer cell lines and inhibits the miR-691 effects on the biological behavior of human pancreatic cancer cell line BXPC-3. Methods miR-691 used real-time fluo- rescent quantitative PCR for detection. Artificial chemical synthesis inhibition of transfected by miR-691 and BXPC-3 cells. Detection of cell apoptosis by flow cytometry,. By Transwel - x room experiment of cell invasion and metastasis detection BXPC-3 cell invasion and metastasis. Structure. Results BXPC-3 for miR-691 in human pancreatic cancer cell line expression; transfection inhibits miR-691, BXPC-3 decline in the miR-691 expression, no significant change in apoptosis （P 〉 0.05）, transfection inhibits the ceils of miR-691 group by Transwel small room above the blank in the number groups and the control group （P 〈 0. 01 ） . Conclusion miR-691 can be expected to be a candidate target for gene therapy of pancreatic cancer.

关 键 词：胰腺癌 微小RNA 侵袭转移 

分 类 号：R735.9[医药卫生—肿瘤]