IL-24可能通过CXCL12/CXCR4信号轴抑制口腔鳞癌细胞迁移及侵袭能力  

IL-24 Might Inhibit Migration and Invasion Abilities of Oral Squamous Cell Carcinoma Cells through CXCL12/CXCR4 Signal Axis

作　　者：金松[1] 田立华[1] 苏勤[1] 郭艳[2] 

机构地区：[1]华中科技大学同济医学院附属普爱医院口腔科,湖北武汉430033 [2]中国医科大学第四医院口腔科,辽宁沈阳110000

出　　处：《口腔医学研究》2017年第5期482-485,共4页Journal of Oral Science Research

基　　金：辽宁省自然科学基金(编号:2013021094)

摘　　要：目的:探讨慢病毒介导IL-24基因转染口腔鳞癌细胞对细胞迁移和侵袭能力的影响及对CXCL12/CXCR4信号轴的影响。方法:培养OSCC细胞株,分为联合处理组、IL-24转染组、抑制剂组和空白对照组。转染后培养48h,ELISA法检测各组细胞液中IL-24表达,检测不同处理组细胞细胞凋亡、迁移和侵袭能力,实时荧光定量PCR和Western blot法检测细胞中IL-24、CXCL12和CXCR4基因和蛋白表达。结果:联合处理组和IL-24转染组细胞上清液中IL-24水平均显著高于抑制剂组和空白对照组(P<0.05);联合处理组迁移数和侵袭数均低于IL-24转染组、抑制剂组和空白对照组,且IL-24转染组和抑制剂组均低于空白对照组(P<0.05);联合处理组和IL-24转染组细胞中IL-24基因和蛋白表达量均高于抑制剂组和空白对照组(P<0.05),联合处理组和IL-24转染组细胞中CXCL12和CXCR4基因和蛋白表达量均低于抑制剂组和空白对照组(P<0.05)。结论:上调IL-24可有效抑制OSCC细胞迁移及侵袭,可能与特异性抑制CXCL12/CXCR4信号通路有关。Objective：To investigate the effect of lentivirus-mediated IL-24 gene transfection on migration and invasion of oral squamous cell carcinoma（OSCC）cells and the effect on CXCL12/CXCR4 axis signal.Methods：OSCC cell lines were cultured and divided into combined treatment group,IL-24 transfected group,inhibitor group,and blank control group.After transfected culture for 48 h,the levels of IL-24 in the cytosol were detected by using ELISA,and cells migration,invasion abilities,and apoptosis in different groups were detected.The expressions of IL-24,CXCL12,and CXCR4 genes and proteins in cells were detected by using real-time quantitative PCR and Western blot.Results：The levels of IL-24 in the supernatants of combined treatment group and IL-24 transfected group were significantly higher than those of inhibitor group and blank control group（P〈0.05）.The numbers of cell migration and invasion in the combined treatment group were less than those in the IL-24 transfected group,inhibitor group,and blank control group,and those in the IL-24 transfected group and inhibitor group were less than those in the blank control group（P〈0.05）.The expression levels of IL-24 mRNA and proteins in the combined treatment group and IL-24 transfected group were higher than those in the inhibitor group and blank control group（P〈0.05）.The expression levels of CXCL12 and CXCR4 genes and proteins in the combined treatment group and IL-24 transfected group were lower than those in the inhibitor group and blank control group（P〈0.05）.Conclusion：Up-regulation of IL-24 could inhibit cell migration and invasion of OSCC.It might relate with the specific inhibiting CXCL12/CXCR4 signaling pathway.

关 键 词：口腔鳞状细胞癌 IL-24 CXCL12/CXCR4信号轴 迁移 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]