银杏叶提取物对成骨细胞分化的影响及相关机制研究  

Effects and Likely Molecular Mechanism of Ginkgo Biloba Extract on Osteogenic Differentiation of Osteoblasts

作　　者：张晓晓[1,2] 李婧[1] 王俊妹[1] 李彤[1] 杨沫扬 吴哲[1,2,3] 

机构地区：[1]吉林大学口腔医学院修复科,吉林长春130021 [2]吉林省牙发育及颌骨重塑与再生重点实验室,吉林长春130021 [3]广州医科大学口腔医学院修复科,广东广州510140

出　　处：《口腔医学研究》2017年第5期491-495,共5页Journal of Oral Science Research

摘　　要：目的:探讨银杏叶提取物(ginkgo biloba extract,GBE)对成骨细胞分化的影响及其可能的相关分子机制。方法:将不同浓度的GBE作用于MG63,通过茜素红染色及碱性磷酸酶(ALP)活性检测判定其对细胞成骨分化能力的影响。RT-PCR检测Runx2(runt-related transcription factor 2)、一型胶原蛋白(COL1A1)、骨钙素(Osteocalcin,OC)及细胞周期蛋白D1(cyclinD1)的表达,Western Blot检测细胞核蛋白β-catenin及cyclinD1的表达。结果:不同浓度的GBE能提高ALP活性及钙结节数量,在浓度为75 mg/L时最为显著;此外,GBE能显著提高Runx2、COL1A1、OC、cyclinD1的mRNA表达及cyclinD1的蛋白表达,同时促进β-catenin蛋白的核内转移;而上述活性均能被DKK1所抑制。结论:银杏叶提取物对成骨细胞的分化具有积极意义,其机制可能与经典Wnt通路的激活有关。Objective：To investigate the effect and possible mechanisms of GBE（ginkgo biloba extract）on the differentiation of osteoblast.Methods：Different concentrations of GBE were applied to the MG63.The osteogenic differentiation capacity was evaluated by Alizarin red and alkaline phosphatase（ALP）activity.RT-PCR was taken to test the expression of COL1A1（type I collagen）,OC（osteocalcin）,Runx2,and cyclinD1.Western blot was adopted to inspect the protein level of cyclinD1 and subcellular localization ofβ-catenin.Results：Incubation of MG63 cell in GBE-supplemented culture medium significantly increased ALP activity,the mRNA expression levels of COL1A1,OC,Runx2,and cyclinD1 as well as the cyclinD1 protein level andβ-catenin nuclear accumulation at day 7.The matrix mineralization was also augmented after 14 days incubation of GBE.And the 75mg/L was the most effective concentration.Importantly,the positive effects of GBE above were abolished by DKK1,a blocker of the Wnt/β-catenin receptor.Conclusion：GBE has a significantly positive effect on the osteogenic differentiation of the MG63 and the 75mg/L was the most effective concentration,in which Wnt/β-catenin signaling pathway may play an important role.

关 键 词：银杏叶提取物 成骨分化 经典Wnt信号通路 成骨细胞 

分 类 号：R285[医药卫生—中药学]