TLR4,NOD2和NLRP3在牙髓炎中的表达  

Expression of TLR4,NOD2 and NLRP3 in Pulpitis

作　　者：何琼琼[1] 杨颖[2] 黄声富[1] 樊明文[1] 

机构地区：[1]武汉大学口腔医学院牙体牙髓科,湖北武汉430079 [2]武汉科技大学附属天佑医院口腔科,湖北武汉430070

出　　处：《口腔医学研究》2017年第5期496-499,共4页Journal of Oral Science Research

基　　金：国家自然科学基金项目(编号:81271190)

摘　　要：目的:分析牙髓炎中TLR4、NOD2和NLRP3的表达特征。方法:收集健康、龋病及牙髓炎的患牙共47颗分为3个组,采用免疫组织化学染色和单色免疫荧光染色方法检测每组牙髓组织中TLR4、NOD2、NLRP3的表达。结果:健康组中TLR4、NOD2和NLRP3仅在成牙本质细胞中少量表达。TLR4、NOD2和NLRP3在牙髓炎组及龋病组表达均明显高于正常组,大量表达于排列紊乱的成牙本质细胞和多种炎症细胞中,而在炎性病灶周边的牙髓成纤维细胞中三者也有表达。结论:本研究表明TLR4、NOD2和NLRP3在炎性牙髓组织中表达同时增高,提示三者均与牙髓炎的进展相关。Objective：To investigate the expression level of TLR4,NOD2 and NLRP3in inflamed human dental pulp.Methods：Forty-seven human third molars were divided into three groups：healthy group,carious group,and pulpitis group.The expressions of TLR4,NOD2 and NLRP3in each group were investigated by immunohistochemistry and immunofluorescence staining.Results：In healthy group,the expressions of TLR4,NOD2,and NLRP3 were only observed in odontoblastic layer.The expression of TLR4,NOD2,and NLRP3 were higher in the caries and pulpitis groups compared with the healthy group.In inflamed dental pulp,TLR4,NOD2,and NLRP3 were detected in odontoblasts,inflammatory cells,and dental pulp fibroblasts.Conclusion：The expressions of TLR4,NOD2,and NLRP3 were simultaneously increased in pulpitis.TLR4,NOD2,and NLRP3 might associate with the progress of pulpitis.

关 键 词：TLR4 NOD2 NLRP3 牙髓炎 

分 类 号：R781.31[医药卫生—口腔医学]