利用潮汕人群第一前磨牙牙本质天冬氨酸消旋化比率推断生物学年龄的研究  

Age Estimation of Chaoshan Population through Aspartic Acid Racemization in Dentin of First Premolar

作　　者：陈仕生[1] 姚小武[1] 于晓军[2] 王典[2] 

机构地区：[1]汕头大学医学院第二附属医院口腔颌面外科,广东汕头515041 [2]汕头大学医学院法医学教研室,广东汕头515041

出　　处：《口腔医学研究》2017年第5期530-533,共4页Journal of Oral Science Research

基　　金：广东省医学科研基金(编号:A2016565)

摘　　要：目的:探讨潮汕人群第一前磨牙牙本质天冬氨酸消旋化比率推断年龄的准确性。方法:收集潮汕人群第一前磨牙标本60例,利用高效液相色谱法检测其牙本质内天冬氨酸消旋化(D/L)比率,并与实际年龄建立回归方程;再用3例另外收集的牙本质标本检验方程的准确性。结果:潮汕人群第一前磨牙牙本质天冬氨酸消旋化(D/L)比率与年龄高度正相关(P<0.001),相关系数r=0.967,消旋化速率k=6.0×10^(-4)/年,推断年龄误差SE=2.60岁。3例验证标本的误差分别是-0.3岁,+1.6岁,-2.2岁。结论:潮汕人群第一前磨牙牙本质天冬氨酸消旋化(D/L)比率可以准确推断年龄。Objective：To analyze the relationship between chronological age and the D/L aspartic acid ratio in dentin of the first premolar of Chaoshan population.Methods：Sixty first premolar teeth from living individuals of known age were retrieved.Dentin was extracted from these teeth.The D-and L-aspartic acids in dentins were separated and detected by high performance liquid chromatography（HPLC）system.Linear regression was performed between the D/L aspartic acid ratio of dentins and chronological age.Dentin from another 3cases was employed to test the accuracy of the regression equation.Results：Linear regression analysis showed a significant correlation between the first premolar dentin D/L aspartic acid ratio and chronological age（P〈0.001）,the correlation coefficient（r）was 0.967,SE=2.60years;the racemization ratio k=6.0×10-4/year.The errors of 3cases were -0.3years,＋1.6years and -2.2years.Conclusion：Aspartic acid racemization in the first premolar dentin can be used as an accurate method to estimate chronological age in the Chaoshan population.

关 键 词：第一前磨牙 天冬氨酸消旋化比率 推断年龄 

分 类 号：R78[医药卫生—口腔医学]