免疫相关基因在健康革胡子鲶不同组织的表达分析  

The Expression of Immune-Related Genes in Healthy Clarias gariepinus Tissues

作　　者：李转转[1] 陈成勋[1] 王晓梅[1] 王妍 王茜[1] 高金伟[1] 张纯玮 何凌霄[1] 

机构地区：[1]天津市水产生态及养殖重点实验室,天津农学院水产学院,天津300384 [2]天津海友佳音生物科技股份有限公司,天津300350

出　　处：《内蒙古大学学报（自然科学版）》2017年第3期297-302,共6页Journal of Inner Mongolia University：Natural Science Edition

基　　金：天津市应用基础与前沿技术研究计划重点项目(15JCZDJC33500);天津市科技计划项目(16ZXZYNC00090)

摘　　要：应用荧光定量PCR法分析抗菌肽hepcidin、c型溶菌酶和MHCⅠ基因在健康革胡子鲶肝胰脏、脾脏、胃、肠道、肾脏、头肾、心脏、脑、鳃、鳃上器官、背部皮肤、背部肌肉和尾鳍13种组织中的表达.结果显示:3种基因在以上组织中均有表达,Hepcidin基因在脾脏中表达最高,显著高于除肾脏以外的其它组织(P<0.05);c型溶菌酶基因在胃中表达量最高,与其余组织的表达差异显著(P<0.05);而MHCⅠ基因在脾脏和鳃中表达量相对较高,但13种组织间的表达差异不显著(P>0.05).The expression levels of hepcidin,c type lysozyme and MHC I genes in hepatopancreas, spleen, stomach, intestines, kidney, head-kidney, heart, brain, gill, suprabranchial organ, dorsal skin, dorsal muscles PCR. The results we tissues. The expressi sues except kidney （ er expressed in the s no significant differe and caudal fins in healthy C. gariepinus were analyzed by real-time quantitative re as follows. The three genes were ubiquitously expressed in thirteen examined on level of hepeidin gene was significantly higher in spleen than in the other tis- P〈0.05）. The expression level of c-type lysozyme gene was significantly high- tomach than other tissues （P〈0.05）. The expression level of MHC I gene had nces in those tissues（P〉0.05）.

关 键 词：革胡子鲶 抗菌肽 c型溶菌酶 主要组织相容性复合体Ⅰ 实时荧光定量PCR 

分 类 号：S965.128[农业科学—水产养殖] Q341[农业科学—水产科学]