正交试验优化提取壮药马尾千金草生物碱  

Optimizing the Extraction of Alkaloids from Lyeopodium sieboldii Miq by Orthogonal Design

作　　者：许丹妮[1,2] 杨海玲[3,4] 许元明[1] 苏秀芳[1] 郑广进[1] 

机构地区：[1]广西民族师范学院化学与生物工程系,广西崇左532200 [2]广西高校桂西南特色植物资源化学重点实验室培育基地,广西崇左532200 [3]广西中医药大学,广西南宁530001 [4]成都中医药大学,四川成都610072

出　　处：《安徽农业科学》2017年第14期115-116,131,共3页Journal of Anhui Agricultural Sciences

基　　金：广西高等学校科学技术研究项目(LX2014170);广西中医药大学中药学博士点建设工程开放课题(201410-14)

摘　　要：[目的]优化马尾千金草生物碱的提取条件。[方法]以马尾千金草的总生物碱含量为指标,运用正交试验设计对酸水浓度、料液比、浸提次数3个因素进行考查,优化最佳提取工艺。[结果]酸水浸泡提取的最佳条件为加入10倍的1%酸水液,浸泡提取3次,每次24 h,生物碱得率为0.0751%,马尾千金草生物碱含量为7.51 mg/g。[结论]该提取方法操作较简单、成本低、生物碱得率高,能有效提取马尾千金草总生物碱。[ Objective ] The research aimed to optimize the extraction condition of alkaloids from Lyeopodium sieboldii. [ Method ] Taking the total alkaloid content of Lyeopodium sieboldii as index,the orthogonal experiment was used to evaluate acid water concentration, solid-liquid ratio and extraction times. The optimum extraction process was optimized. [ Result ] The optimum condition of the acid water extraction was 1% acid water solution with 10 times, soaking and extracting for 3 times, each time 24 h, the total alkaloids was 0. 0751%, the alkaloid content of Lyeopodium sieboldii was 7.51 mg/g. [ Conclusion] The extraction method is simple,low operation cost, high rate of alkaloids, which can effectively extract the total alkaloids of Lyeopodium sieboldii.

关 键 词：马尾千金草 总生物碱 正交试验 优化提取 

分 类 号：S567.23[农业科学—中草药栽培]